KMB17 Human Diploid Cell Line

From 4-week-old girl embryos, established in 1975 by Kunming Institute of Medical Biology, Chinese Academy of Medical Sciences; It can be used as a transfection host for the study of lung injury or differentiation. It was identified as free of mycoplasma/STR and put into storage.

Animal species/Organism girl

Tissue and Cell Type Embryos

Morphology fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

80% MEM (MD0300)+10% newborn bovine serum+10% hydrolyzed milk protein+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0308)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=6459859

Guo R., Cao Y.-Y., Dai Z.-Z., Qu S.-R., Zhuang J.-Y.

Characteristics of a human diploid cell line, KMB-17.

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 3:226-230(1981）