pgsA-745 China hamster ovarian cell-Xylosyltransferase I deficiency strain

Chinese hamster ovarian cells (xylotransferase I deficiency type) pgsA-745, also known as PgsA-745, pgsA745, CHO-745, Mutant 745, etc. This cell is a xylyltransferase-deficient mutant treated with a mutagenic agent (ethyl mesylate) of CHO-K1 cells and screened. In 1957, T. T. Puck and others established a spontaneous immortalized cell line from the living ovarian tissue of adult Chinese hamsters, naming it CHO cells, and the CHO-K1 cells are precisely a subclone of CHO. Because the most primitive CHO cells are proline-deficient and require proline addition to the culture system to support their growth (F-12K medium already contains 0.6 mM L-proline), all known CHO cell lines retain this trait. These cells are easily transfected and secrete very few endogenous proteins, making them widely used for in vitro expression of recombinant proteins and for viral infection experiments.

Animal Species:Chinese hamster

Tissue Source:Ovaries; Disease: Normal cells

Morphology:epithelial, grows adherently

Culture Medium Formula:F12K Complete Medium（MD0401）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=3858816; DOI=10.1073/pnas.82.10.3197; PMCID=PMC397742
Jeffrey David Esko, Tod E. Stewart, William H. Taylor;
Animal cell mutants defective in glycosaminoglycan biosynthesis.
Proc. Natl. Acad. Sci. U.S.A. 82:3197-3201(1985)