C8-B4 Mouse microglia

Mouse microglia C8-B4, also known as C8B4.
C8-B4 cells are isolated and formed from the cerebellum of 8-day-old mice and spontaneously immortalized in vitro tissue culture. They are a cell line with astrocyte or microglial characteristics. After primary isolation for four months, these cells exhibit various morphologies and slow proliferation, then gradually enrich to form a homogeneous cell population. These cells express classic microglial markers (such as MAC1, F4/80, 2-4G2), and since they do not express differentiation antigens present in the early stages of monocyte lineages, they appear to originate from identified microglial precursors. Additionally, C8-B4 cells synthesize CD4 molecules and produce and release large amounts of glutamic acid. These cells can be used for immunology and neuroscience research.

Animal Species: Mouse

Tissue Source:Brain, cerebellum; Disease: Normal; spontaneously manifesting eternal life

Morphology:Neuron-like, adherent growth

Culture Medium Formula:DMEM (high Sugar) Complete Medium（MD0101）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=8873987; DOI=10.1016/0165-3806(96)00101-0
Francoise Alliot, Marie-Chantal Marty, Danielle Cambier, Bernard Pessac;
A spontaneously immortalized mouse microglial cell line expressing CD4.
Brain Res. Dev. Brain Res. 95:140-143(1996)

PubMed=21961028; DOI=10.2174/1877381801002010148; PMCID=PMC3180920
Jessica M. Crain, Jyoti J. Watters;
Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.
Open Drug Discov. J. 2:148-167(2010)