FL83B Normal liver cells of mice

Normal mouse liver cells FL83B, also known as FL-83B. This cell was isolated in 1969 by Waymouth C and colleagues at Jackson Lab from 15-17 day mouse embryonic livers as a spontaneous immortalized cell line. Reportedly, the cell karyotype is aneuploid, with a chromosome mode of 75 (between 65-79) and a polyploid rate of 22%. Additionally, these cells express cholesterol and glycogen, can store glycogen, actively synthesize cholesterol, and produce serum proteins.

Animal Species:Mouse

Tissue and Cell Type: Liver; Illness: Normal

Morphology: epithelial, grows adherently

Medium Formulation:

F12K (MD0400)+10% fetal bovine serum (SD0200)+1% GA (SD0038)

Link to complete medium: F12K complete medium (MD0401)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

CLPUB00349
Charity Waymouth, Harry W. Chen, Bonnie G. Wood;
Characteristics of mouse liver parenchymal cells in chemically defined media.
In Vitro 6:371.1-371.1(1971)

PubMed=4572697; DOI=10.1016/0014-4827(73)90089-X
Jan Leslie Breslow, Howard R. Sloan, Victor Joaquim Ferrans, J.L. Anderson, Robert Isaac Levy;
Characterization of the mouse liver cell line FL83B.
Exp. Cell Res. 78:441-453(1973)

PubMed=26280412; DOI=10.1038/nbt.3327
Sean J. Humphrey, Seyed Babak Azimifar, Matthias Mann;
High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics.
Nat. Biotechnol. 33:990-995(2015)