R1 Mouse embryonic stem cells

The R1 cell line was established in August 1991, using 3.5-day blastocysts obtained from the mating of two 129 sublines (129S1/SvImJ and 129X1/SvJ). R1 pluripotency was initially detected by tetraploid embryos, though diploid embryos can also be used. However, when tetraploid aggregate detection was performed, about 20% of subclones from the 14th generation showed the original developmental potential of R1. Animals derived from R1 are fertile once they reach adulthood. Injecting genetically modified R1 cells into C57 blastocysts or aggregating them with CD-1 or outbred ICR 8-cell stage embryos can produce efficient germline transfer. Over 90% of knockout clones enter the germline through aggregated chimeras (n> 60). Each batch passed mycoplasma testing in the database and was negative. During culture, Kunming MEF should be used as the culture layer cells. When frozen, it is P24 generation.

Animal Species:Mouse

Tissue Source: Cell clusters inside mouse embryos

Morphology:Spherical clones, growing close to walls

Culture Medium Formula:STEMCult ™ ES Culture Medium (XF)（P2601-M）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.