Nanog-GFP Mouse embryonic stem cells expressing

Nanog-GFP cells were constructed by Hatano et al. in 2005. Through homologous recombination, 2.5 kb upstream and 3.9 kb downstream fragments of Nanog ORF were selected as homologous arms, and the Nanog gene on one chromosome was replaced with the GFP gene (GFP-IRES-PURO-PA cassette), thereby constructing heterozygous mES cells driven by the Nanog promoter to express GFP. These mES cells express GFP when undifferentiated, but do not express GFP after differentiation. Each cell volume is 1×106。 Each batch passed mycoplasma testing in the database and was negative.

Animal Species:Mouse

Tissue Source: Cell clusters inside mouse embryos

Morphology:Spherical clones, growing close to walls

Culture Medium Formula:STEMCult ™ ES Culture Medium (XF)（P2601-M）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.