LL/2 (LLC1) Mouse lung cancer cells (Lewis)

LL/2 (LLC1) cells are derived from the Lewis lung cancer cell line of C57BL mice, established from implantation of primary Lewis lung cancer in mouse lungs. This cell line is widely used as a metastasis model and is employed to study the mechanisms of action of cancer chemotherapy drugs. (1) Tubergenicity: High tumorigenicity in C57BL mice. (2) Antigen expression: H-2b. (3) Resistance: Resistant to 1,3-bis(2-chloroethyl)-1-nitrosourea, but sensitive to methotrexate. (4) Metastatic capability: Reported to have high tumorigenicity but weak metastasis in mice. The LL/2 (LLC1) cell line is widely used for its value in cancer research, especially in studying tumor metastasis, tumor angiogenesis, and evaluating the effectiveness of chemotherapy drugs.

Animal Species: Mouse

Tissue Source:Lewis lung cancer

Morphology:Round - lightly attached to the wall or suspended;

Culture Medium Formula:DMEM (high Sugar) Complete Medium（MD0101）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=7226139; DOI=10.1016/0304-3835(80)90130-5
John S. Bertram, Przemyslaw Janik;
Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture.
Cancer Lett. 11:63-73(1980)

DOI=10.4051/ibc.2010.2.4.0014
Kaori Yoshino, Kaoru Saijo, Chikako Noro, Yukio Nakamura;
Development of a simple method to determine the mouse strain from which cultured cell lines originated.
Interdisciplinary Bio Cent. 2:14.1-14.9(2010)

PubMed=28599420; DOI=10.3892/ol.2017.5877; PMCID=PMC5453008
Vaidotas Stankevicius, Karolina Kuodyte, Diana Schveigert, Danute Bulotiene, Tomas Paulauskas, Kristina Daniunaite, Kestutis Suziedelis;
Gene and miRNA expression profiles of mouse Lewis lung carcinoma LLC1 cells following single or fractionated dose irradiation.
Oncol. Lett. 13:4190-4200(2017)

PubMed=30084848; DOI=10.1038/sdata.2018.157; PMCID=PMC6080492
Heiko Schuster, Wen-Guang Shao, Tobias Weiss, Patrick Gino Angelo Pedrioli, Patrick Roth, Michael Weller, David S. Campbell, Eric W. Deutsch, Robert L. Moritz, Oliver Planz, Hans-Georg Rammensee, Ruedi Aebersold, Etienne Caron;
A tissue-based draft map of the murine MHC class I immunopeptidome.
Sci. Data 5:180157.1-180157.11(2018)

PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Jamie L. Almeida, Aleksandra Dakic, Karin Kindig, Maikan Kone, Deborah Lee Dormady Letham, Scott Langdon, Ruth Peat, Jayamalini Holding-Pillai, Erin M. Hall, Mark Ladd, Megan D. Shaffer, Heath Berg, Jin-Liang Li, Georges Wigger, Steve Lund, Carolyn R. Steffen, Barbara B. Fransway, Bob Geraghty, Manuela Natoli, Beth A. Bauer, Susanne M. Gollin, Dale W. Lewis, Yvonne A. Reid;
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:e0218412.1-e0218412.24(2019)