alpha TC1 clone 6 Alpha cells of the mouse pancreas

alpha TC1 clone 6 is a α cell line isolated in 1988 from the pancreas of mice with adenomas. It was cloned from transgenic mice expressing the SV40 large T antigen oncogene in adenomas produced under the control of the rat glucagon protopromoter. alpha TC1 clone 6 is a pancreatic alpha cell line. It is cloned from the alpha TC1 cell line, which was obtained from adenocarcinoma produced in transgenic mice transfected with the SV40 macro T antigen anti-cancer gene controlled by the rat pre-hypercacagin promoter. Its parent cell line is poorly differentiated and produces both hyperglycin and insulin. The two clonal cell lines, alpha TC1 clone 6 and alpha TC1 clone 9, are highly differentiated and produce only hypercagon. Alpha TC1 clone 6 exhibits the highest degree of differentiation and expresses the highest levels of hypercagon. The alpha TC1 clone 6 is useful for studying many biological characteristics of the islet, including hyperglycin biosynthesis and cytokinin sensitivity.

Animal Species:Mouse

Tissue Source:Pancreas

Morphology:Epithelial cells, adherent growth; Loosely connected clusters and suspended single cells

Culture Medium Formula:MEM Complete Medium（MD0301）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=2108069; DOI=10.2337/diab.39.4.415
Kazuyuki Hamaguchi, Edward H. Leiter;
Comparison of cytokine effects on mouse pancreatic alpha-cell and beta-cell lines. Viability, secretory function, and MHC antigen expression.
Diabetes 39:415-425(1990)

PubMed=34871457; DOI=10.1111/bph.15769
Jian-Xin Jia, Qi Kang, Shun-Zhi Liu, Ya-Bin Song, Florence Susan Wong, Ying-Kun Qiu, Ming-Yu Li;
Artemether and aspterric acid induce pancreatic alpha cells to transdifferentiate into beta cells in zebrafish.
Br. J. Pharmacol. 179:1962-1977(2022)