MKN74 [MKN-74] Human gastric cancer cells

MKN74 (also known as MKN-74; MKN 74) was isolated from liver metastases in a 37-year-old male patient with differentiated tubular adenocarcinoma in the stomach. This cell is nearly subdiploid karyotype, with chromosome number drift visible in later passage; p53 is mostly wild-form, but some batches have reported I251L point mutations; RUNX3 mRNA deletion; It expresses synuclein-γ (SNCG) and enteromorphic differentiation markers (CK8/18, CEA, EpCAM), and can form tumor balls without serum, making it a moderately differentiated adenocarcinoma cell. It is commonly used in stem cell research for gastric cancer.

Animal Species: Human

Tissue Source:Gastric tubular adenocarcinoma

Morphology:Epithelial cells, adherent growth

Culture Medium Formula:RPMI-1640 Complete Medium（MD0201）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

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