MKN1 [MKN-1] Human gastric cancer cells

In 1975, Hojo and colleagues isolated primary cells from the gastric lymph node metastases of a 72-year-old Japanese male gastric cancer patient, cultured them in vitro and passed them off, and established an adult gastric cancer cell line, naming it MKN-1. The primary lesion pathology is diagnosed as gastric adenosquamous cell carcinoma. MKN-1 cells also have the potential for bidirectional differentiation into glandular and squamous epithelium, making it a rare mixed subtype of gastric cancer. It is widely used in research on the mechanisms of gastric cancer development, drug screening, and in vivo model construction.

Animal Species: Human

Tissue Source:Gastric adenosquamous cell carcinoma; Stomach, metastasis of lymph nodes

Morphology:Epithelial cells, adherent growth

Culture Medium Formula:RPMI-1640 Complete Medium（MD0201）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

CLPUB00581
Haruto Hojo;
Establishment of cultured cell lines of human stomach cancer origin and their morphological characteristics.
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CLPUB00584
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