NCI-H146 [H146] Small cell lung cancer cells

Cells express relatively large amounts of c-myc mRNA, but the c-myc DNA sequence does not amplify. It expressed c-myb, v-fms, Ha-ras, Ki-ras, N-ras, and c-raf 1 mRNAs. These cells are highly expressing four biochemical markers: neuron-specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase, and bombesin-like immunoreactivity. Additionally, these cells grow in large amounts of suspended clumps, making survival rates impossible to measure. During routine culture, the culture medium usually contains a large amount of cell debris that is almost impossible to remove, which is normal. Bispecific antibodies can also be added to the medium to prevent bacterial contamination.

Animal Species: Human

Tissue Source:Lungs; Originates from metastatic lesions: bone marrow

Morphology:Suspension, multiple cells clustered together

Culture Medium Formula:RPMI-1640 Complete Medium（MD0201）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

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