GM12878 Human immortalized lymphoblasts

GM12878 cells were obtained from B lymphocytes from peripheral venous blood of a Caucasian woman, and human immortalized lymphoblasts obtained after EB virus transformation. Our library confirmed a base mutation (G→A) at nucleotide position 681 of exon 5 CYP2C19 gene in this cell, resulting in an abnormal splicing site. This abnormal splicing site causes exon 5 of the gene to lose 40 bp of nucleotides from positions 643 to 682, resulting in amino acid deletions at positions 215 to 227. Truncated CYP2C19 proteins have only 234 amino acids and lose catalytic activity due to the lack of heme binding regions.

Animal Species:Human

Tissue Source:Peripheral blood, B lymphocytes

Morphology:Round and clustered, growing suspended

Culture Medium Formula:RPMI-1640 Complete Medium(MD0202)

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

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