MP41 Human uveal melanoma cells

Human uveal melanoma cells MP41 is an epithelioid cell line isolated in 2006 from the primary uveal tumor of a 49-year-old woman diagnosed with melanoma. The cell replication time is about 41 hours, with a mutation occurring at base 626 of the oncogene GNA11 c.626a A>T. Uveal melanoma is the most common primary malignant eye tumor in adults. Currently, there is no standard adjuvant therapy to prevent metastasis, and there are no effective treatments after metastasis. The primary tumor formed by this cell may partially reflect the molecular mechanisms of uveal melanoma in terms of genetic variation and mutation. It has been reported that the mTOR pathway is activated in most uveal melanoma cell lines and is independent of AKT signaling. The mTOR inhibitor Everolimus can reduce the survival rate of uveal melanoma cell lines and significantly delay tumor growth in four types of uveal melanoma PDX mice.

Animal Species: Human 

Tissue Source:Eyes, uveitis,Melanoma

Morphology:Epithelial cells, adherent growth

Culture Medium Formula:RPMI-1640 Complete Medium（MD0203）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=24994677; DOI=10.1016/j.molonc.2014.06.004; PMCID=PMC5528590
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Establishment of novel cell lines recapitulating the genetic landscape of uveal melanoma and preclinical validation of mTOR as a therapeutic target.
Mol. Oncol. 8:1508-1520(2014)

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Protein and mRNA expression in uveal melanoma cell lines are related to GNA and BAP1 mutation status.
Invest. Ophthalmol. Vis. Sci. 65:37-37(2024)