hAGHESC-2 Solitary, male haploid embryonic stem cells

By using micromanipulation, maternal pre-pronuclei from fertilized eggs were removed to obtain solitary male haploid blastocysts, which were then established in vitro as haploid embryonic stem cell lines. 

Animal Species:Human

Tissue Source: inner cell mass 

Morphology:Clonal

Culture Medium Formula:STEMCult ™ ES Culture Medium (XF)（P2601）

Cells used in the feeding layer: E12.5 MEF（ICR MEF）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.