MDOK Renal epithelial cells

The MDOK cell line, also known as Madin-Darby Ovine Kidney cells, is a spontaneously immortalized epithelial cell line isolated from renal tissue of an adult male sheep. These cells are highly susceptible to various viruses, including vesicular stomatitis virus, bovine infectious rhinotracheitis virus, and type 2 blue tongue virus, making them an ideal host for vaccine development. Additionally, they serve as a vital tool in virology, veterinary medicine, toxicology, and cell biology research.

Animal Species:Sheep

Tissue Source: kidney

Morphology:Epithelioid in appearance, adherent growth

Culture Medium Formula:MEM Complete Medium（MD0301）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=13567776; DOI=10.3181/00379727-98-24111
Stewart Harvey Madin, Norman B. Darby Jr.;
Established kidney cell lines of normal adult bovine and ovine origin.
Proc. Soc. Exp. Biol. Med. 98:574-576(1958)

PubMed=14282199; DOI=10.1007/BF00320563
Walter Anthony Nelson-Rees, Alexis John Kniazeff, Norman B. Darby Jr.;
Peculiarities of ovine cells in culture.
Chromosoma 16:79-89(1965)

PubMed=5587551; DOI=10.1159/000129961
Walter Anthony Nelson-Rees, Alexis John Kniazeff, Norman B. Darby Jr.;
Debut and accumulation of centric fusion products: an index to age of certain cell lines.
Cytogenetics 6:436-450(1967)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)