HCC827-GFP-LUC

This cell line was created in March 1994. It was derived from a female patient with a lung adenocarcinoma with an acquired mutation in the EGFR tyrosine kinase domain (E746-A750 deletion). The patient smoked 1 pack of cigarettes per month between the ages of 25 and 26. She stopped smoking 12 years before diagnosis. This is the labeled Luc cell line.

Luciferases are oxidases that produce light in the presence of a substrate (D-luciferin) in an organism, a process known as bioluminescence. The most common luciferase genes come from a family of light-producing enzymes called firefly luciferases 1 and 2.

In preclinical research, small animal live imaging plays a vital role in visualizing physiological processes, disease progression, and therapeutic development. Luciferase (luc)-activated cell lines provide a simple, high-throughput, and robust method for quantitatively assessing tumor burden and tumor response to therapy in test animals by bioluminescence imaging (BLI)1,2. (This cell is dual-labeled with GFP and LUC)

Tissue and Cell Type: Lung adenocarcinoma

Morphology: epithelial cell-like, adherent growth

Complete Growth Medium and Culture Conditions:

90%1640(MD0200)+10%fetal bovine serum(SD0200)+G/A(SD0038)

Complete medium: 1640 complete medium (Cat. No.: MD0201)

Gas phase: air, 95%; carbon dioxide, 5%. Temperature: 37 degrees Celsius

Complete freezing medium: Program-free cell freezing medium (low serum) (SD0001)

Note: This product is for scientific research use only