PC-3-luc

Human prostate cancer cells, PC-3, were derived from bone marrow metastases in a 62-year-old white male patient with grade IV prostate cancer. The cells showed low expression of acid phosphatase and testosterone-5-alpha reductase activity.

This is a labelled Luc cell line, if the fluorescence expression is not strong, 2ug/ml puromycin can be used to screen for 48 hours and then replaced with a normal culture system, the floating cells are negative cells that do not express LUC, and the change of fluid can be discarded.

Luciferase is an oxidase that glows in the presence of a substrate (D-luciferin) in living organisms, a process known as bioluminescence. The most common luciferase genes are from a family of light-producing enzymes called firefly luciferase 1 and 2. 

In preclinical studies, small animal live imaging plays a crucial role in visualising physiological processes, disease progression and therapeutic development. Luciferase (luc) activated cell lines by bioluminescence imaging (BLI)1,2 provide a simple, high-throughput and robust method for quantitatively assessing tumour burden and tumour response to therapy in subject animals.

Tissue source/Issue and Cell Type: Prostate; from metastases: bone

Morphology: Epithelioid, adherent；The cells grow in soft agar as nests and can be adapted to suspension cultures

Medium Formulation:

90% F12K (MD0400)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: F12K complete medium  MD0401)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Declaration: This product is for scientific research only.