Opt™HighPoly transfection reagent

Synthetic from polymers such as PEI, without animal components, suitable for gene transfection mediated by retroviruses and lentiviruses in various cells. This reagent has lower toxicity than Polybrene and higher transfection efficiency.

Cell infection:

Inoculate cells at a density of approximately 150000 cells per well into a 6-well cell culture plate, with 2 ml of normal culture medium per well

2. Divide the virus solution and dilute the lentivirus with calculated MIO value to complete culture medium. Add the final concentration of adherent cells (5ug/ml Polybrene) and suspended cells (8ug/ml Polybrene) and mix well

3. Add well mixed virus infection medium to each well of a well plate with a cell growth density of about 30-40%.

4. Gently shake the board to mix cells and viruses.

5. Incubate the board in a 37 ° C, 5% CO2 environment and infect for 2-3 days

6.Stable conversion: Remove the old culture medium after 48-72 hours. Wash twice with PBS, add screening medium for cell screening and cloning, transient expression: remove growth medium and add fresh growth medium. Harvest cells 24-72 hours later.

Note: The product is for scientific research purposes only