LMH cells

LMH is a primary hepatocellular carcinoma epithelial cell line established in 1981 by Tomoyuki Kitagawa at the Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo, Japan.

Comments

Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.

The morphology of the cells is dendritic like.

The cells express glucose-6-phosphatase and weak canalicular ATPase activity.

The cells form tumors in athymic nude mice.

LMH cells contain a low level of functional estrogen receptor and are inducible for the expression of the liver specific apolipoprotein II (apoII) gene.

The cell line is useful for transfection studies.

NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.

To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid.

Vessels prepared in this fashion may be used immediately.

PubMed=3607775

Kawaguchi T., Nomura K., Hirayama Y., Kitagawa T.

Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH.

Cancer Res. 47:4460-4464(1987)

PubMed=2330000; DOI=10.1210/mend-4-2-201

Binder R., MacDonald C.C., Burch J.B.E., Lazier C.B., Williams D.L.

Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line.

Mol. Endocrinol. 4:201-208(1990)

PubMed=2352324; DOI=10.1128/JVI.64.7.3249-3258.1990

Condreay L.D., Aldrich C.E., Coates L., Mason W.S., Wu T.-T.

Efficient duck hepatitis B virus production by an avian liver tumor cell line.

J. Virol. 64:3249-3258(1990)

PubMed=10564780; DOI=10.1016/s0304-4165(99)00159-2

Kolluri S., Elbirt K.K., Bonkovsky H.L.

Heme biosynthesis in a chicken hepatoma cell line (LMH): comparison with primary chick embryo liver cells (CELC).

Biochim. Biophys. Acta 1472:658-667(1999)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022

Karger A., Bettin B., Lenk M., Mettenleiter T.C.

Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.

J. Virol. Methods 164:116-121(2010)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5

Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.

High-throughput sequencing for species authentication and contamination detection of 63 cell lines.

Sci. Rep. 11:21657-21657(2021)