Suspension™ BHK Medium

Suspension™ BHK series suspension medium is a medium that supports BHK cell suspension culture. It is composed of more than 100 small molecule compounds. It does not contain protein, but contains a small amount of hormones. It uses ferric citrate and other methods to replace transferrin in cell growth. For applications, using the Suspension™ series of suspension cell culture media, the maximum cell density that can be cultured is above 10×10^6 cells/ml. The serum-free and protein-free components can maintain no differences between batches, and the use of small molecule compounds can maintain Expression of normal proteins for cell growth.

Medium composition: 500ml Suspension™ BHK series suspension medium (Qida Bio: SP02), 5ml G/A (Qida Bio: SD0038) 

Operation steps: 

The adaptive training method of cells from conventional culture medium to serum-free medium is based on There are 2 types of cells: 

For suspension cells (it takes 2-6 weeks):
1. Start adaptive training when the cell density reaches the maximum;
2. Add serum-free medium to make the difference between serum-free medium and normal medium The ratio becomes 1/2 of the original
3. When the cell density reaches the maximum, add serum-free medium to make the cell density 1/5 of the original;
4. Culture the cells. If the cells reach the maximum density, the survival rate of the cells is greater than 85%, and the time to reach maximum density is similar to before, then the cells can continue adaptive training; if the cells show slow growth or reduced survival rate, then steps 2-3 need to be repeated 3 times;
5. Serum-free can be gradually increased The ratio of medium until cells can grow completely in serum-free medium.

For adherent cells (it takes 2-6 weeks):
1. When the cells reach the maximum density, start adaptive training;
2. Digest the adherent cells with trypsin, centrifuge at 350×g for 5 minutes to collect the cells, and use serum-free solution containing 5% serum. Resuspend the culture medium to make the cell density reach 4×10^5 cells/ml.
3. Culture the cells until they reach the maximum density;
4. Repeat steps 2 - 3 and add 2%, 1%, 0.5% and 0.1% serum to the serum-free medium in sequence for cell culture; if the survival rate of the cells is lower than 80%, then it is necessary to return to the content of the previous serum, or adopt a more detailed decreasing content.
5. Culture cells in serum-free medium until completely.

Disclaimer: Products are for scientific research use only