Suspension™ 293 (XF) Medium

Suspension™ 293 (XF) suspension medium is a serum-free and animal component-free medium composed of more than 100 small molecule compounds. It is suitable for 293 series suspension culture of cells and large-scale reactor culture. The medium does not contain protein. Adding a small amount of hormone can maintain normal protein expression content in cells, with stable components and no batch differences.

Medium composition: 500ml Suspension™ 293 (XF) suspension medium (Qida Bio: SP03), 5ml G/A (Qida Bio: SD0038) 

Operation steps: 

Adaptive training of cells from conventional culture medium to serum-free medium The method is divided into 2 types according to the type of cells: 

For suspension cells (it takes 2-6 weeks):
1. Start adaptive training when the cell density reaches the maximum;
2. Add serum-free medium to make the serum-free medium and normal culture The proportion of base becomes 1/2 of the original.
3. When the cell density reaches the maximum, add serum-free medium to make the cell density become 1/5 of the original;
4. Culture the cells. If the cells reach the maximum density, the survival of the cells will increase. If the rate is greater than 85%, and the time to reach maximum density is similar to before, then the cells can continue adaptive training; if the cells show slow growth or reduced survival rate, then steps 2-3 need to be repeated 3 times;
5. It can be gradually improved Serum-free medium until cells can grow completely in serum-free medium.

For adherent cells (it takes 2-6 weeks):
1. When the cells reach the maximum density, start adaptive training;
2. Digest the adherent cells with trypsin, centrifuge at 350×g for 5 minutes to collect the cells, and use serum-free solution containing 5% serum. Resuspend the culture medium to make the cell density reach 4×10^5 cells/ml.
3. Culture the cells until they reach the maximum density;
4. Repeat steps 2 - 3 and add 2%, 1%, 0.5% and 0.1% serum to the serum-free medium in sequence for cell culture; if the survival rate of the cells is lower than 80%, then it is necessary to return to the content of the previous serum, or adopt a more detailed decreasing content.
5. Culture cells in serum-free medium until completely.

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