KTC-1 LUC thyroid cancer cells

The luciferase reporter gene system is a report system that uses luciferin as the substrate to detect the luciferase activity of firefly, and the biological fluorescence released during the reaction between the enzyme and the substrate reflects the biological activity of the enzyme. Firefly luciferase gene is used as a reporter gene to indicate the gene expression under different treatment conditions. Firefly luciferase, with a size of 61 KDa and a single subunit protein, can catalyze the oxidation of luciferin to produce oxyluciferin; The strongest luminescence wavelength of luciferin catalyzed by firefly luciferase is 560 nm. Usually, the 5 ´ UTR or promoter of the target gene is cloned to the upstream of Firefly Luciferase, or the 3 ´ UTR or expected RNAi target sequence is cloned to the downstream of Firefly Luciferase, and the transcriptional regulation of the promoter or regulatory element is detected by detecting the reaction activity of firefly luciferase with the substrate. Renilla Luciferase was used as an internal reference to eliminate the differences between experimental groups such as cell number or transfection efficiency. KTC-1 luc labeled cells, labeled with firefly luciferase, are often used for small animal imaging.

Organizational source/Issue and Cell Type: Thyroid cancer

Morphology: Polygonal or irregular shapes, growing against walls

Complete Growth Medium and Culture Conditions：

90%RPMI-1640（MD0200）+10%的FBS(SD0200)和1%G/A(SD0038)

Freezing solution: 55% basic culture medium+40% FBS+5% DMSO Temperature: liquid nitrogen

Gas phase: air, 95%; CO2, 5% temperature: 37 ℃

Statement: The product is for scientific research only