T2(174 x CEM.T2) human lymphoblastic cells

This cell line is a variant of T1 cell line (ATCC CRL-1991). By selecting SFR1-MI.3 monoclonal antibody (needle On HLA DR). The cell line is a suitable transfection host. T1 series and T2 series are used for research Investigate antigen processing and T cell recognition of class I MHC antigen. The cells do not express HLA-DR, and class II major histocompatibility (MHC) antigen negative. The cells aggregate and grow but do not express HLA B5 and HLA-DR. Compared with T1, T2 seems to be lost Two copies of chromosome 6 derived from CEMR. 3 were lost. Class II major histocompatibility (MHC) antigen was negative.

Species: Human

Tissue and Cell Type: Lymphoma

Morphology: circular, suspended growth

Morphology: epithelial-like, adherent growth

Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% Fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: RMPI1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

cryopreservation :  cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=3872841; DOI=10.1007/BF00375376

Salter R.D., Howell D.N., Cresswell P.

Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids.

Immunogenetics 21:235-246(1985)

PubMed=1373173

Riberdy J.M., Cresswell P.

The antigen-processing mutant T2 suggests a role for MHC-linked genes in class II antigen presentation.

J. Immunol. 148:2586-2590(1992)