MeT-5A human membrane mesothelial cells

Mesothelial cells were isolated from pleural effusion of non-cancer patients, and pRSV-T plasmid (a sv40ori construct containing SV40 early region and long terminal repeat sequence of Rous sarcoma virus) was transfected into cells and cloned. The cells did not have tumorigenicity in immunosuppressed mice, but formed colonies in semi-solid medium.

Vimentin, keratin and sv40t antigen were positive. The cells can be used to screen chemical and biological agents with growth factors, carcinogens, mutagens and other activities.

Animal species/Organism Human

Tissue and Cell Type: Mesothelium

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

ECGM endothelial cell culture medium (product No.: P1001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution:non-programmed cooling cell cryopreservation solution (low serum) (SD0001) 

Note: This product is for scientific research only

Patent=US4885238

Reddel R.R., Yang K., Rhim J.S., Brash D.E., Su R.T., Lechner J.F., Gerwin B.I., Harris C.C., Amstad P.

Immortalized human bronchial epithelial mesothelial cell lines.

Patent number US4885238, 05-Dec-1989

PubMed=2541616

Ke Y., Reddel R.R., Gerwin B.I., Reddel H.K., Somers A.N., McMenamin M.G., LaVeck M.A., Stahel R.A., Lechner J.F., Harris C.C.

Establishment of a human in vitro mesothelial cell model system for investigating mechanisms of asbestos-induced mesothelioma.

Am. J. Pathol. 134:979-991(1989)

PubMed=9533946; DOI=10.1165/ajrcmb.18.4.2943

Kahlos K., Anttila S., Asikainen T., Kinnula K., Raivio K.O., Mattson K., Linnainmaa K., Kinnula V.L.

Manganese superoxide dismutase in healthy human pleural mesothelium and in malignant pleural mesothelioma.

Am. J. Respir. Cell Mol. Biol. 18:570-580(1998)

PubMed=15920167; DOI=10.1016/S0002-9440(10)62492-3

Gordon G.J., Rockwell G.N., Jensen R.V., Rheinwald J.G., Glickman J.N., Aronson J.P., Pottorf B.J., Nitz M.D., Richards W.G., Sugarbaker D.J., Bueno R.

Identification of novel candidate oncogenes and tumor suppressors in malignant pleural mesothelioma using large-scale transcriptional profiling.

Am. J. Pathol. 166:1827-1840(2005)

PubMed=17331233; DOI=10.1186/1471-2164-8-62

Nymark P., Lindholm P.M., Korpela M.V., Lahti L., Ruosaari S., Kaski S., Hollmen J., Anttila S., Kinnula V.L., Knuutila S.

Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines.

BMC Genomics 8:62-62(2007)

PubMed=22576795; DOI=10.3892/or.2012.1763

Ashinuma H., Takiguchi Y., Kitazono S., Kitazono-Saitoh M., Kitamura A., Chiba T., Tada Y., Kurosu K., Sakaida E., Sekine I., Tanabe N., Iwama A., Yokosuka O., Tatsumi K.

Antiproliferative action of metformin in human lung cancer cell lines.

Oncol. Rep. 28:8-14(2012)

PubMed=24926545; DOI=10.1097/JTO.0000000000000202

Riquelme E., Suraokar M.B., Rodriguez J., Mino B., Lin H.Y., Rice D.C., Tsao A., Wistuba I.I.

Frequent coamplification and cooperation between C-MYC and PVT1 oncogenes promote malignant pleural mesothelioma.

J. Thorac. Oncol. 9:998-1007(2014)

PubMed=26011428; DOI=10.1111/cas.12698

Hakiri S., Osada H., Ishiguro F., Murakami H., Murakami-Tonami Y., Yokoi K., Sekido Y.

Functional differences between wild-type and mutant-type BRCA1-associated protein 1 tumor suppressor against malignant mesothelioma cells.

Cancer Sci. 106:990-999(2015)

PubMed=28553954; DOI=10.1038/onc.2017.147

Kato T., Sato T., Yokoi K., Sekido Y.

E-cadherin expression is correlated with focal adhesion kinase inhibitor resistance in merlin-negative malignant mesothelioma cells.

Oncogene 36:5522-5531(2017)