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Current position:Home > Products > highQu GmbH > PCR & qPCR > Standard & Direct PCR > PCRbeam™ Rapid PCR Test Kit
  • PCRbeam™快速PCR检测试剂盒

PCRbeam™ Rapid PCR Test Kit

No.:PDK0101

Price: ¥1673.00

specification:
50 tests
amount: - +
  • PCRbeam™快速PCR检测试剂盒
Products Description

highQu PCRbeam ™ Rapid PCR detection kit is a convenient tool for rapid detection of gene-specific amplification products obtained by PCR, LAMP or RPA. Detection is based on the immune response driven by biotin and fluorescein isothiocyanate (FITC), so the amplified DNA should include biotin and FITC tags. PCR amplification requires a primer 5 'end labeling FITC and a primer 5' end labeling biotin. Alternatively, a probe labeled with gene-specific FITC or biotin can replace the primer of one of the markers. The kit includes pcr - beam coated with biotin ligand ™ The membrane strip is coated with biotin ligand on the detection strip and anti-rabbit antibody on the control strip. The bottom of the strip used for sample application contains anti-fitc antibody attached to the gold particles. pccrbeam ™ The test buffer is tris buffer saline test.

PCRbeam ™ The rapid PCR detection kit can be used for established tests or self-made analysis as a fast and sensitive yes/no detection method. The detection sensitivity is up to 100% higher than that of an achievable gel with ethidium bromide staining to provide environmentally friendly, economical and economical alternatives to the use of mutagens for staining.

In order to ™ Before testing, establish sensitive PCR-based testing. We recommend using hot start PCR enzyme or main mixture, such as highQu ALLin ™ Hot start Taq Mastermix or ALLin ™ Hot start Taq DNA polymerase.

Product application:

Low-flux PCR, LAMP, RPA detection

Sensitive detection of specific amplification products

Fast and 20 times more sensitive alternative EtBr staining gel

Economic alternatives based on qPCR detection

Product advantages:

Sensitive detection of specific products of PCR, LAMP and RPA genes

No gel loading and ethidium bromide treatment after PCR

Compared with the detection method based on qPCR, the cost is saved

Fast and simple procedures, saving time

Soak the membrane strip in the detection buffer of mixed PCR products for 10 minutes. A transverse sample flow driven by gold particles moves the solution upward. The FITC-labeled DNA chain is bound to the anti-FITC antibody on the gold particle, and the biotin-labeled DNA chain is captured on the detection band with biotin ligand. When the two DNA strands remain hybridized at room temperature, the test band will form a polymer with red and blue color. The surplus gold particles not captured by FITC move upward, and the anti-FITC antibody and anti-rabbit antibody combine to form a red-blue control band.

If there is no PCR product in the reaction, only the control band can be seen. If there is a specific product, the test strip will also have color.




Statement: The product is for scientific research only


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