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Current position:Home > Products > highQu GmbH > RT-PCR & RT-qPCR > Reverse Transcription & RT-PCR > HighScriber™ reverse transcriptase
  • HighScriber™ 逆转录酶

HighScriber™ reverse transcriptase

No.:RTM0301

Price: ¥2653.00 ¥9233.00

specification:
10000 u
amount: - +
  • HighScriber™ 逆转录酶
Products Description

HighScriber ™ Reverse transcriptase Mix is an advanced tool that can efficiently reverse transcript up to 12-15 kb of cDNA. Mixing includes HighScriber ™ Reverse transcriptase and ribonuclease inhibitors are preserved, and have strong cDNA synthesis and convenient use. HighScriber ™ Reverse transcriptase allows high detection sensitivity from 1 pg of total RNA. The general reaction temperature range (38 ° C - 55 ° C) ensures efficient synthesis of cDNA from complex or GC-rich templates.

The enzyme takes ssRNA or ssDNA as a template and has no detectable RNA-specific ribonuclease H activity in RNA-dNA hybridization. A highly reduced activity of ribonuclease H allows transcription of transcripts with a long total length. HighScriber ™ Reverse transcriptase can be used in RACE because it has terminal transferase activity - adding nucleoside at the 3 'end of cDNA.

Ribonuclease inhibitor premixed RT ensures that RNA is protected from ribonuclease degradation.

Provide 5X ALLin ™ HighScriber buffer, including everything needed for cDNA synthesis reaction. In order to reduce the liquid transfer step, it contains MgCl2, dNTPs, reinforcing agent and stabilizer. The only thing to add is template RNA and primer.

The best RT activity is observed at 45-50 ° C

Temperature range 38-55 ° C

RT inactivation at 85 ° C for 10 minutes

Definition unit:

Using poly (rA) oligo (dT) 18 as a template, 1 nmol dTTP was added to acid-insoluble substances within 10 minutes at 37 ° C, with a total reaction volume of 50 μ l。


Product application:

CDNA synthesis of long transcripts up to 15 kb

Template generation of RT-PCR and RT-qPCR

Synthesis of cDNA from composite template

Product advantages

Efficient synthesis of cDNA by mixing thermostable reverse transcriptase and ribonuclease inhibitor

High-yield full-length transcripts up to 12-15kb

Synthesis of cDNA from composite template at up to 55 ℃

High sensitivity detection of 1pg total RNA template




Statement: The product is for scientific research only.


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