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Current position:Home > Products > highQu GmbH > PCR & qPCR > Standard & Direct PCR > One-step quantitative PCR kit
  • 一步法定量PCR试剂盒

One-step quantitative PCR kit

No.:RTK0201

Price: ¥7315.00

specification:
100 r of 50 µl
amount: - +
  • 一步法定量PCR试剂盒
Products Description

HighQu 1Step RT PCR Kit combines 20X RT and RNase inhibitor mixed with effective reverse transcription and 2X PCR Mastermix for subsequent cDNA amplification in the same tube.

RT2 Mix, 20X is a mixture of engineering MMuLV (stable at 40-55 ° C, allowing high production of long transcripts) and effective ribonuclease inhibitor, which protects template RNA from RNA enzyme.

The obtained cDNA was then amplified by one-step RT PCR Mastermix, 2X. PCR mastermix contains our proprietary hot start Taq DNA polymerase. Small molecular inhibitors inhibit the activity of the enzyme at room temperature. The enzyme is only active after heating, which allows highly specific and sensitive amplification without primer dimer formation and background. Combined with optimized buffer, the enzyme provides higher success rate and requires PCR application, such as complex amplification or longer template and rapid cycle.

Hot start Taq DNA polymerase has the same PCR accuracy as Taq DNA polymerase, 4.5 x 10 ^ 4 (nucleotides merged before the error occurred), and produces a - tail product suitable for connecting to TA cloning vector

For maximum convenience, the kit even includes PCR water setting reaction, so the only thing you need to do is high-quality RNA template.


Product application:

One-step RT-PCR

RT-PCR Study of Complex GC/AT Enrichment Template

Rapid RT-PCR


Product advantages

Including RT-Mix and RT-PCR Mastermix for RT and PCR in a test tube

RT mixture contains RNase inhibitor and heat-resistant RT (up to 55 ° C)

RT-PCR-Mastermix allows low copy number target detection



Statement: The product is for scientific research only.


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