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  • ALLin™ Mega HS HiFi DNA 聚合酶

ALLin™ Mega HS HiFi DNA polymerase

No.:HLE0401

Price: ¥1449.00 ¥6895.00

specification:
100 u
amount: - +
  • ALLin™ Mega HS HiFi DNA 聚合酶
Products Description

From our HiFi polymerase, highQu ALLin ™ Compared with Taq, ALLin ™ Ultra high fidelity DNA polymerase provides a much lower error rate of PCR, with a fidelity of up to 100%. Compared with Mega HiFi, this hot-start enzyme version can achieve higher PCR sensitivity and specificity as well as room temperature reaction settings, which is an excellent choice for multiple reactions. Allen ™ Mega HS HiFi DNA polymerase is designed faster and can produce up to 20 kb long PCR products from complex GC-rich templates. Therefore, ALLin ™ Ultra high fidelity DNA polymerase is an excellent choice for longer and very complex PCR applications requiring the highest fidelity. It is the preferred enzyme for cloning and sequencing applications including NGS. The resulting flat-terminal PCR product is suitable for connecting to the flat-terminal vector. In order to improve the ligation efficiency, it is recommended to use the repair kit (HER0101) for modification.

In order to maximize convenience, ALLin ™ Mega HS HiFi Mastermix, 2X (HLM0401) and ALLin ™ Mega HS HiFi Red Mastermix, 2X (HLM0501) are available.


Product application:

Sequencing, including NGS library preparation

Hot start PCR, multiplex

Rapid high-fidelity PCR (up to 100 x Taq)

Long PCR up to 20 kb

Amplification of complex (GC/AT-rich) templates

Blunt-end cloning and other applications


Product advantages:

Hot start enzyme can improve sensitivity and obtain excellent multiple results

Fast, high yield PCR, 100 times higher fidelity than Taq

Even 20 kb PCR from complex templates

Improve continuous synthesis capability to achieve faster amplification and higher yield

High thermal stability can better denature the template rich in GC

The best choice for NGS library preparation and other sequencing applications

5x ALLin ™ The buffer includes the optimal amount of Mg2+and dNTP



Statement: The product is for scientific research only


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