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  • ALLin™超高保真Red预混合液

ALLin™ Ultra High Fidelity Red Premix

No.:HLM0301

Price: ¥2233.00 ¥10073.00

specification:
100 r of 50 µl
amount: - +
  • ALLin™超高保真Red预混合液
Products Description

From our HiFi polymerase, highQu ALLin ™ Compared with Taq, ALLin ™ Ultra high fidelity DNA polymerase (article number: HLE0301) can provide much lower error rate PCR, with a fidelity of up to 100%. ALLin ™ The design speed of ultra-high fidelity DNA polymerase is faster, and it can produce up to 20 kb long PCR products from complex GC-rich templates. So ALLin ™ Ultra high fidelity DNA polymerase is the best choice for ultra-long or very complex PCR applications that require the highest fidelity. It is the preferred enzyme for cloning and sequencing applications including NGS. The resulting flat-terminal PCR product is suitable for connecting to the flat-terminal vector. However, in order to improve the efficiency of terminal connection, it is recommended to use the repair kit (HER0101) for the final modification of the end of PCR fragments.

ALLin ™ The ultra-high fidelity Red premix maximizes the use of 2X Red Mastermix, Mega HiFi DNA polymerase, and provides the advantage of reducing liquid transfer and direct gel loading. ALLin ™ Mega HiFi Red Mastermix, 2X, pre mixed with red dye and density reagent, can be directly loaded onto gel after PCR. In 2% agarose TAE gel, the dye migrates with~350 bp DNA, and in 1% agarose TAE gel with~600 bp DNA fragments. The main mixture even provides PCR water, and the only template to be added is the template with primer.


Product application:

Rapid high-fidelity PCR (up to 100 x Taq)

Long PCR up to 20 kb

Direct gel loading option

Amplification of complex (GC/AT-rich) templates

Sequencing, including NGS

Inert cloning and other applications


Product advantages:

Fast, high yield PCR, 100 times higher fidelity than Taq

Even 20 kb PCR from complex templates

Improve continuous synthesis capability to achieve faster amplification and higher yield

High thermal stability can better denature the template rich in GC

The best choice for NGS and other sequencing applications

Red main mixture is directly loaded with gel for water supply



Statement: The product is for scientific research only.


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