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Current position:Home > Products > highQu GmbH > Enzymes & Cloning > RNA-related Enzymes > Taq-DNA polymerase
  • Taq-DNA聚合酶

Taq-DNA polymerase

No.:PCE0201

Price: ¥3493.00 ¥6293.00

specification:
1500 u
amount: - +
  • Taq-DNA聚合酶
Products Description

HighQu-Taq DNA polymerase is a classic enzyme in conventional PCR applications, providing a high amplification rate of 3-5 kb target under various conditions. Taq-DNA polymerase was purified from recombinant Escherichia coli strains carrying Taq-DNA polymerase gene.

Taq-DNA polymerase is a thermostable 5 ´ → 3 ´ DNA polymerase. Lack of 3 ´ → 5 ´ exonuclease (proofreading) activity, and 5 ´ → 3 ´ exonuclease activity is low. Polymerase showed deoxynucleotidyltransferase activity, resulting in the A-hanging of the 3 'end of PCR product and allowing TA cloning. The PCR accuracy of Taq-DNA polymerase was 4.5x104 (the number of nucleotides before the first error).

The reaction buffer provided is the most flexible in optimization and does not contain dNTPs and magnesium. 50 mM MgCl2 solution is provided separately, allowing magnesium optimization from 1 mM to 4 mM final concentration as required. Complete or mixed dNTPs can be purchased separately from highQu.


Product application:

Conventional PCR can reach 5 kb under normal and fast conditions

RT-PCR, clone PCR, TA clone

Library construction, genotyping and screening


Product advantages:

High yield rate under conventional PCR and rapid cycle conditions

Strong performance under various conditions.

Excellent performance on complex (GC rich) templates



Statement: The product is for scientific research only


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