ORA™ ROX L real-time qPCR probe Mix

highQu ORA ™ ROX L real-time qPCR probe mixture is based on small-molecule inhibitor technology. Hot start PCR allows the highest sensitivity and specificity under standard and rapid qPCR cycle conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and ensuring rapid expansion, to achieve detection of minimum or non-optimal early Ct values.

ORA ™ ROX L real-time qPCR probe mixture provides an additional advantage and simplifies the tracking process, because they are colored with inert blue dye, so that the sample can be better visible during the liquid transfer and treatment process.

Our mastermix provides PCR water to ensure optimal performance. In order to adapt to a wide range of instruments, ORA ™ QPCR probe mastermix has three versions - no ROX, low or high ROX concentration.

ROX passive reference dye premixed in ROX L and ROX H qPCR can be used alternatively. If the buyer has an optional ROX detection instrument and wants to carry out optional standardization of the signal, the user must select this option in the software.

Note to the Buyer: When purchasing this product, it does not involve the United States patent: 5928907 and corresponding patents outside the United States.

Product application:

QPCR with instrument calibrated with low ROX concentration

QPCR detection based on specific probes:

TaqMan ®， Molecular beacon, scorpion ™ probe

Gene expression analysis and quantification:

GDNA, cDNA, viral DNA, low copy number gene

Product advantages:

Universal - standard and fast cycle, all probes qPCR detection

GC or rich template of qPCR, single plex and multiplex

Rapid expansion, early Ct

Inert blue dye for better sample visibility and tracking

Statement: The product is for scientific research only

1. Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology BMC Genomics, 2017 Nov 3 Luciana Fernandes Brito, Marta Irla, Jörn Kalinowski et al. … inserts were amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…

2.  Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum Marine Drugs, 2016 Jun 30 Nadja A. Henke, Sabine A. E. Heider, Petra Peters-Wendisch et al. amplified …using All-in HiFi polymerase (highQu, Kraichtal, Germany)...

3.  Methanol-based γ-aminobutyric acid (GABA) production by genetically engineered Bacillus methanolicus strains Industrial Crops and Products, 2017 Nov 1 Marta Irla, Ingemar Nærdal, Trygve Brautaset et al. …The DNA amplification was carried out with ALLin™ HiFi DNA Polymerase (highQu)…

4.  Genome-Based Genetic Tool Development for Bacillus methanolicus:Theta- and Rolling Circle-Replication Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production Frontiers in Microbiology, 2016 Sep 22 Marta Irla, Tonje M. B. Heggeset, Ingemar Nærdal, Lidia Paul, Tone Haugen et al. … inserts with ALLinTM HiFi DNA Polymerase (highQu) or the ExpandTM High Fidelity PCR System (Roche)….

5. Programming biological operating systems: Genome design, assembly and activation Nature Methods Vol 11, 2014 Daniel G Gibson …amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…

6.  Genomic, transcriptomic and phenotypic analysis of the plant growth promoting and phosphate solubilizing Paenibacillus riograndensis SBR5 Dissertation, Bielefeld University, 2017 Jun Luciana Fernandes de Brito …inserts were amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…

7.  Role of adhesin proteins in Chlamydia infection Dissertation, Heinrich Heine University Düsseldorf, 2017 Feb Alison Favaroni …PCR with All-in HiFi Polymerase (highQu)…

8.  Charakterisierung der pH abhängigen Konformation und Multimerisierung des Influenza-A-Virus M1-Proteins Dissertation, Universität zu Lübeck, 2016 Jun Jens Klingbeil … wurde die PCR-Ansätze mit der ALLinTM HiFi DNA Polymerase (highQu) nach Herstellerangaben durchgeführt…

9.  Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum Genes, Basel, April 2018 Nadja A. Henke, Julian Wichmann, [...], and Volker F. Wendisch CR-generated fragments (All-in HiFi, highQu, Kraichtal, Germany) were assembled into isolated plasmids

10. Variation in ectomycorrhizal fungal communities associated with Silver linden (Tilia tomentosa) within and across urban areas FEMS Microbiology Ecology, 94, 2018 Maarten Van Geel, KangYu, Tobias Ceulemans 1U HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and 1 μL genomic DNA.

11.  Characterization of D-arabitol as newly discovered carbon source of Bacillus methanolicus Frontiers in microbiology, 2019 M Gil López, M Irla, LF Brito Inserts were amplified by polymerase chain reactions (PCRs) using ALLin TM HiFi DNA Polymerase (HighQu)

12.  Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas … Front. Bioeng. Biotechnol., 26 September 2019 M Mindt, S Hannibal… PCR-fragments were amplified using ALLin™ HiFi DNA Polymerase (highQu GmbH)

13.  Xylose as preferred substrate for sarcosine production by recombinant Corynebacterium glutamicum Bioresource technology, 2019 M Mindt, M Heuser amplified using ALLin™ HiFi DNA Polymerase (highQu GmbH)

14. Forest edge effects on the mycorrhizal communities of the dual-mycorrhizal tree species Alnus glutinosa (L.) Gaertn. Science of the Total Environment, 2019 M Boeraeve, O Honnay, ALLin HiFi Buffer and 0.25 μL ALLin HiFi DNA Polymerase (HighQu, Kraichtal, Germany

15.  Soil organic matter rather than ectomycorrhizal diversity is related to urban tree health PloS one, 2019 M Van Geel, K Yu, G Peeters 1× HiFi Buffer, 1U HiFi DNA Polymerase (HighQu, Kraichtal, Germany

16. 16. Fermentative Production of N-Alkylated Glycine Derivatives by Recombinant Corynebacterium glutamicum Using a Mutant of Imine Reductase DpkA From … 2019 - ir.niist.res.in KM Nampoothiri, M Heuser ALLin TM HiFi DNA Polymerase (highQu GmbH, Kraichtal, Germany).

17. Improved Astaxanthin Production with Corynebacterium glutamicum by Application of a Membrane Fusion Protein Marine drugs, 2019 NA Henke… amplified by a high-fidelity PCR (Allin HiFi, highQu, Kraichtal, Germany)

18.  Charting the metabolic landscape of the facultative methylotroph Bacillus methanolicus bioRxiv, 2019 B Delépine, MG Lopez (PCRs) with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany)

19. Metabolic engineering of Corynebacterium glutamicum towards methanol-dependent growth 20182019 - pub.unibielefeld.de G Hennig PCR to amplify DNA-fragments for cloning was performed with Allin HiFi polymerase (HighQu, Kraichtal, Germany) according to the manufacturer’s protocol.

20.  The impact of forest fragmentation on mycorrhizal communities Dissertation, 2019 Margaux Boeraeve DNA. ALLin HiFi Buffer and 0.25 μL ALLin HiFi DNA Polymerase (HighQu, Kraichtal, Germany).

21.  SRSF7 maintains its homeostasis through the expression of Split-ORFs and nuclear body assembly Nature Structural & …, 2020 V Königs, COF Machado The proofreading thermostable ALLin RPH Polymerase (highQu) was used for the amplification of PCR fragments for cloning.

22.  Carbon Metabolism of Methylotrophic Methanogens and Asgard Archaea in Marine Sediments Dissertation, Uni Bremen, 2019 X Yin PCR was conducted with ALLin RPH polymerase Kit (highQu, Kraichtal, Germany)

Product Insert ORA™ SEE qPCR Probe ROX L Mix, 2X

MSDS ORA™ SEE qPCR Probe ROX L Mix, 2X