ALLin™ Hot Start Taq Premix

highQu ALLin ™ Hot start taq dna polymerase is an excellent sensitive enzyme, whose room temperature activity is blocked by small molecular inhibitors. This enzyme becomes active only after heating, because it allows highly specific and sensitive amplification without the formation and background of primer dimer.

ALLin combined with optimization ™ Buffer enzymes have a high success rate in demanding PCR applications such as amplification of complex or long templates and rapid circulation.

highQu ALLin ™ Hot start Taq-DNA polymerase has the same PCR accuracy as Taq-DNA polymerase, i.e. 4.5 × 104 (nucleotides added before the error occurred), and produce the A-tail product suitable for connecting to the TA cloning vector.

highQu ALLin ™™ Hot start Taq DNA polymerase is to maximize the use of 2X masterbatch to provide additional advantages, reduce liquid transfer and minimize errors. Mastermix even provides PCR water, which is the only template with primer to be added.

Applicable to:

Hot start PCR up to 6 kb, low copy target detection

PCR of complex (GC rich) template

Rapid PCR, multiplexing, TA cloning

Product advantages:

High yield, no background standard or rapid PCR

In long (6 kb) or rich GC template, it is successful in rough sample PCR

ALLin ™ Mastermix provides PCR water

Statement: The product is for scientific research only

1. Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology BMC Genomics, 2017 Nov 3 Luciana Fernandes Brito, Marta Irla, Jörn Kalinowski et al. … inserts were amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…

2.  Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum Marine Drugs, 2016 Jun 30 Nadja A. Henke, Sabine A. E. Heider, Petra Peters-Wendisch et al. amplified …using All-in HiFi polymerase (highQu, Kraichtal, Germany)...

3.  Methanol-based γ-aminobutyric acid (GABA) production by genetically engineered Bacillus methanolicus strains Industrial Crops and Products, 2017 Nov 1 Marta Irla, Ingemar Nærdal, Trygve Brautaset et al. …The DNA amplification was carried out with ALLin™ HiFi DNA Polymerase (highQu)…

4.  Genome-Based Genetic Tool Development for Bacillus methanolicus:Theta- and Rolling Circle-Replication Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production Frontiers in Microbiology, 2016 Sep 22 Marta Irla, Tonje M. B. Heggeset, Ingemar Nærdal, Lidia Paul, Tone Haugen et al. … inserts with ALLinTM HiFi DNA Polymerase (highQu) or the ExpandTM High Fidelity PCR System (Roche)….

5. Programming biological operating systems: Genome design, assembly and activation Nature Methods Vol 11, 2014 Daniel G Gibson …amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…

6.  Genomic, transcriptomic and phenotypic analysis of the plant growth promoting and phosphate solubilizing Paenibacillus riograndensis SBR5 Dissertation, Bielefeld University, 2017 Jun Luciana Fernandes de Brito …inserts were amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…

7.  Role of adhesin proteins in Chlamydia infection Dissertation, Heinrich Heine University Düsseldorf, 2017 Feb Alison Favaroni …PCR with All-in HiFi Polymerase (highQu)…

8.  Charakterisierung der pH abhängigen Konformation und Multimerisierung des Influenza-A-Virus M1-Proteins Dissertation, Universität zu Lübeck, 2016 Jun Jens Klingbeil … wurde die PCR-Ansätze mit der ALLinTM HiFi DNA Polymerase (highQu) nach Herstellerangaben durchgeführt…

9.  Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum Genes, Basel, April 2018 Nadja A. Henke, Julian Wichmann, [...], and Volker F. Wendisch CR-generated fragments (All-in HiFi, highQu, Kraichtal, Germany) were assembled into isolated plasmids

10. Variation in ectomycorrhizal fungal communities associated with Silver linden (Tilia tomentosa) within and across urban areas FEMS Microbiology Ecology, 94, 2018 Maarten Van Geel, KangYu, Tobias Ceulemans 1U HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and 1 μL genomic DNA.

11.  Characterization of D-arabitol as newly discovered carbon source of Bacillus methanolicus Frontiers in microbiology, 2019 M Gil López, M Irla, LF Brito Inserts were amplified by polymerase chain reactions (PCRs) using ALLin TM HiFi DNA Polymerase (HighQu)

12.  Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas … Front. Bioeng. Biotechnol., 26 September 2019 M Mindt, S Hannibal… PCR-fragments were amplified using ALLin™ HiFi DNA Polymerase (highQu GmbH)

13.  Xylose as preferred substrate for sarcosine production by recombinant Corynebacterium glutamicum Bioresource technology, 2019 M Mindt, M Heuser amplified using ALLin™ HiFi DNA Polymerase (highQu GmbH)

14. Forest edge effects on the mycorrhizal communities of the dual-mycorrhizal tree species Alnus glutinosa (L.) Gaertn. Science of the Total Environment, 2019 M Boeraeve, O Honnay, ALLin HiFi Buffer and 0.25 μL ALLin HiFi DNA Polymerase (HighQu, Kraichtal, Germany

15.  Soil organic matter rather than ectomycorrhizal diversity is related to urban tree health PloS one, 2019 M Van Geel, K Yu, G Peeters 1× HiFi Buffer, 1U HiFi DNA Polymerase (HighQu, Kraichtal, Germany

16. 16. Fermentative Production of N-Alkylated Glycine Derivatives by Recombinant Corynebacterium glutamicum Using a Mutant of Imine Reductase DpkA From … 2019 - ir.niist.res.in KM Nampoothiri, M Heuser ALLin TM HiFi DNA Polymerase (highQu GmbH, Kraichtal, Germany).

17. Improved Astaxanthin Production with Corynebacterium glutamicum by Application of a Membrane Fusion Protein Marine drugs, 2019 NA Henke… amplified by a high-fidelity PCR (Allin HiFi, highQu, Kraichtal, Germany)

18.  Charting the metabolic landscape of the facultative methylotroph Bacillus methanolicus bioRxiv, 2019 B Delépine, MG Lopez (PCRs) with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany)

19. Metabolic engineering of Corynebacterium glutamicum towards methanol-dependent growth 20182019 - pub.unibielefeld.de G Hennig PCR to amplify DNA-fragments for cloning was performed with Allin HiFi polymerase (HighQu, Kraichtal, Germany) according to the manufacturer’s protocol.

20.  The impact of forest fragmentation on mycorrhizal communities Dissertation, 2019 Margaux Boeraeve DNA. ALLin HiFi Buffer and 0.25 μL ALLin HiFi DNA Polymerase (HighQu, Kraichtal, Germany).

21.  SRSF7 maintains its homeostasis through the expression of Split-ORFs and nuclear body assembly Nature Structural & …, 2020 V Königs, COF Machado The proofreading thermostable ALLin RPH Polymerase (highQu) was used for the amplification of PCR fragments for cloning.

22.  Carbon Metabolism of Methylotrophic Methanogens and Asgard Archaea in Marine Sediments Dissertation, Uni Bremen, 2019 X Yin PCR was conducted with ALLin RPH polymerase Kit (highQu, Kraichtal, Germany)