2BS Human Embryo Lung Diploid Cells

It is from a girl embryo and was established by the Beijing Institute of Biological Products in 1976. It was identified as free of mycoplasma/STR and put into storage.

Tissue and Cell Type: embryonic lung, diploid cell

Morphology: Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

80% MEM (MD0300)+10% newborn bovine serum FBS (SD0200)+10% hydrolyzed milk protein+1% GA (SD0038)

Link to complete medium: MEM complete medium (MD0308)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0100)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=1055416; DOI=10.1073/pnas.72.4.1441

Martin G.R., Evans M.J.

Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro.

Proc. Natl. Acad. Sci. U.S.A. 72:1441-1445(1975)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)