S-180 Mouse Ascites Tumor Cells

It was obtained from CFW mice. It can form tumor in vivo and is widely used in tumor chemotherapy experiment.

Tissue and Cell Type: ascites tumor

Morphology: rotundity Half pasted and half suspended

Culture media and additives/Complete Growth Medium and Culture Conditions:

RPMI 1640（MD0200） +10%FBS（SD0200）+1%GA(SD0038)

Link to complete medium: RPMI1640 complete medium (MD0218)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

PubMed=13035452; DOI=10.1093/jnci/13.5.1299

Dunham L.J., Stewart H.L.

A survey of transplantable and transmissible animal tumors.

J. Natl. Cancer Inst. 13:1299-1377(1953)

PubMed=13823721

Foley G.E., Drolet B.P., McCarthy R.E., Goulet K.A., Dokos J.M., Filler D.A.

Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines.

Cancer Res. 20:930-939(1960)

PubMed=14185313; DOI=10.1126/science.146.3641.241

Cell Culture Collection Committee

Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.

Science 146:241-243(1964)

PubMed=14221803

Foley G.E., Drolet B.P.

Loss of neoplastic properties in vitro. I. Observations with S-180 cell lines.

Cancer Res. 24:1461-1467(1964)

PubMed=366289

Abercrombie M., Turner A.A.

Contact reactions influencing cell locomotion of a mouse sarcoma in culture.

Med. Biol. 56:299-303(1978)

PubMed=1546443; DOI=10.1016/0165-2427(92)90107-2

Alfaro G., Lomeli C., Ocadiz R., Ortega V., Barrera R., Ramirez M., Nava G.

Immunologic and genetic characterization of S180, a cell line of murine origin capable of growing in different inbred strains of mice.

Vet. Immunol. Immunopathol. 30:385-398(1992)