HK-2 Human Renal Epithelial Cells

It is reported that human renal proximal convoluted tubule cell HK-2 contains a recombinant retroviral vector pLXSN 16 E6/E7 containing HPV-16 E6/E7 gene. Although pLXSN 16 E6/E7 is resistant to neomycin, G418 cannot be used to isolate the transduced clone. The cell line can be separated into single cells based on Southern and FISH analysis. The E6/E7 gene was detected in the HK-2 genome by PCR. The cells retained well differentiated PTCs phenotype. Alkaline phosphatase γ Glutamyl transpeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin α 3、 β The expression of integrin and fibronectin was positive. The expression of factor VIII associated antigen, 6.19 antigen and CALLA endopeptidase was negative. HK-2 cells retain the functional characteristics of proximal renal tubular epithelial cells, such as Na+dependent/phlorizin-sensitive glucose transport and adenylate cyclase response to parathyroid gland, but are not sensitive to antidiuretic hormone. This cell has the ability to generate and store glycogen. HK-2 cells grew adherent to the wall, did not grow on methylcellulose and soft agar, and could not grow in suspension.

Tissue and Cell Type: kidney, cortex/proximal convoluted tubule

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

K-SFM (Invitrogen, 17005-042) culture solution contains two components: one bottle of basic culture solution (article No. 10724-011)+Growth factor 2 tube (article number 37000-015).

Note 2: According to the instructions of K-SFM culture solution, Gentamicin Solution needs to be added additionally. Gentamicin is recommended/mphotericin Solution 500 × （Gibco, R-015-10）。

Complete medium: KGM cell medium (article No.: P8001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

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