MR1 [derivative Of HB-11048] Chinese Hamster X Mouse B Lymphocyte Hybridoma

Animals were immunized with mouse T cells (Th1). Spleen cells fuse with NS-1 myeloma cells. A 39KDa antibody protein was detected on activated mouse T cells. The culture submitted to ATCC in May 1992 contaminated mycoplasma. The offspring were treated with BM cyclin for 21 days to eliminate mycoplasma. Through mycoplasma detection in this library.

Animal species/Organism Chinese hamster X mouse

Tissue and Cell Type B lymphocyte hybridoma

Morphology lymphoblasts; Suspension growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+ β- Mer 0.05mM（SD0031）+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0206)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

Patent=US5683693

Noelle R.J., Durie F.H., Parker D.C., Appel M.C., Phillips N.E., Mordes J.P., Grenier D.L., Rossini A.A.

Method for inducing T cell unresponsiveness to a tissue or organ graft with anti-CD40 ligand antibody or soluble CD40.

Patent number US5683693, 04-Nov-1997