HMEC-1 Human Microvascular Endothelial Cell Line

Vascular endothelial cell (EC) is located between plasma and vascular tissue. It can not only complete the metabolic exchange of plasma and tissue fluid, but also synthesize and secrete a variety of bioactive substances to ensure the normal contraction and relaxation of blood vessels, play a special role in maintaining vascular tension, regulating blood pressure, blood coagulation and anticoagulation balance, and thus maintain the normal flow of blood and long-term patency of blood vessels. Endothelialization on the surface of anticoagulant materials can reduce the formation of thrombus and platelet activation. Endothelial cells can now be used to study: vasoconstriction and vasodilation, thus controlling blood pressure; Coagulation, anticoagulation and fibrinolysis (thrombosis and fibrinolysis); Arteriosclerosis; Angiogenesis; Inflammation and swelling (edema). Endothelial cells also control some substances, such as white blood cells, entering and leaving blood vessels. In some organs, there are some highly differentiated endothelial cells responsible for special filtration functions. Examples include glomerular and cerebrovascular barriers.

Morphology: endothelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

Endothelial cell culture medium ECGM (product number: P1001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

Patent=WO1992017569A1

Ades E.W., Lawley T.J., Candal F.J.

Immortalization of endothelial cells.

Patent number WO1992017569A1, 15-Oct-1992

PubMed=1361507; DOI=10.1111/1523-1747.ep12613748

Ades E.W., Candal F.J., Swerlick R.A., George V.G., Summers S., Bosse D.C., Lawley T.J.

HMEC-1: establishment of an immortalized human microvascular endothelial cell line.

J. Invest. Dermatol. 99:683-690(1992)

PubMed=12453433; DOI=10.1006/mvre.2002.2434

Unger R.E., Krump-Konvalinkova V., Peters K., Kirkpatrick C.J.

In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.

Microvasc. Res. 64:384-397(2002)