HacaT Human Immortalized Keratinocytes

This cell is the first immortalized epidermal cell derived from normal adult skin tissue and can be used for the study of keratinization of human epidermal cells. Through mycoplasma detection in this library. Through STR detection in this library.

Morphology: epithelial-like; Adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

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Boukamp P., Dzarlieva-Petrussevska R.T., Breitkreutz D., Hornung J., Markham A., Fusenig N.E.

Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.

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Schurer N., Kohne A., Schliep V., Barlag K., Goerz G.

Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes.

Exp. Dermatol. 2:179-185(1993)

PubMed=8504475; DOI=10.1093/carcin/14.5.833

Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.

p53 mutations in human immortalized epithelial cell lines.

Carcinogenesis 14:833-839(1993)

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Screening the p53 status of human cell lines using a yeast functional assay.

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Henseleit U., Zhang J., Wanner R., Haase I., Kolde G., Rosenbach T.

Role of p53 in UVB-induced apoptosis in human HaCaT keratinocytes.

J. Invest. Dermatol. 109:722-727(1997)

PubMed=10523843; DOI=10.1038/sj.onc.1202934

Boukamp P., Popp S., Bleuel K., Tomakidi E., Burkle A., Fusenig N.E.

Tumorigenic conversion of immortal human skin keratinocytes (HaCaT) by elevated temperature.

Oncogene 18:5638-5645(1999)

PubMed=11583982; DOI=10.1016/S0002-9440(10)62541-2

Mueller M.M., Peter W., Mappes M., Huelsen A., Steinbauer H., Boukamp P., Vaccariello M., Garlick J., Fusenig N.E.

Tumor progression of skin carcinoma cells in vivo promoted by clonal selection, mutagenesis, and autocrine growth regulation by granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor.

Am. J. Pathol. 159:1567-1579(2001)

PubMed=15389883; DOI=10.1002/jcb.20212

Lemaitre G., Lamartine J., Pitaval A., Vaigot P., Garin J., Bouet S., Petat C., Soularue P., Gidrol X., Martin M.T., Waksman G.

Expression profiling of genes and proteins in HaCaT keratinocytes: proliferating versus differentiated state.

J. Cell. Biochem. 93:1048-1062(2004)

PubMed=20097197; DOI=10.1016/j.yexcr.2010.01.020

Rasanen K., Vaheri A.

Proliferation and motility of HaCaT keratinocyte derivatives is enhanced by fibroblast nemosis.

Exp. Cell Res. 316:1739-1747(2010)

PubMed=21269460; DOI=10.1186/1752-0509-5-17

Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.

Initial characterization of the human central proteome.

BMC Syst. Biol. 5:17-17(2011)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690

Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.

Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.

Clin. Cancer Res. 17:7248-7264(2011)

PubMed=26989177; DOI=10.1242/dev.133728

Elbediwy A., Vincent-Mistiaen Z.I., Spencer-Dene B., Stone R.K., Boeing S., Wculek S.K., Cordero J., Tan E.H., Ridgway R., Brunton V.G., Sahai E., Gerhardt H., Behrens A., Malanchi I., Sansom O.J., Thompson B.J.

Integrin signalling regulates YAP and TAZ to control skin homeostasis.

Development 143:1674-1687(2016)

PubMed=29660373; DOI=10.1016/j.bbagen.2018.04.012

Touat-Hamici Z., Bulteau A.-L., Bianga J., Jean-Jacques H., Szpunar J., Lobinski R., Chavatte L.

Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines.

Biochim. Biophys. Acta 1862:2493-2505(2018)

PubMed=31629070; DOI=10.1016/j.tiv.2019.104666

da Silva A.C.G., Chialchia A.R., de Castro E.G., e Silva M.R.L., Arantes D.A.C., Batista A.C., Kitten G.T., Valadares M.C.

A new corneal epithelial biomimetic 3D model for in vitro eye toxicity assessment: development, characterization and applicability.

Toxicol. In Vitro 62:104666-104666(2019)