EJ-1 Human B-cell Lymphoma

This is a cell line contaminated by T-24 cells, and the sequence variation is homozygous hrasp; From a 82-year-old woman. Through mycoplasma detection in this library. Through STR detection in this library.

Morphology: lymphoblasts; Suspension growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4

Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.

Screening the p53 status of human cell lines using a yeast functional assay.

Mol. Carcinog. 19:243-253(1997)

DOI=10.11418/jtca1981.18.4_329

Tanabe H., Takada Y., Minegishi D., Kurematsu M., Masui T., Mizusawa H.

Cell line individualization by STR multiplex system in the cell bank found cross-contamination between ECV304 and EJ-1/T24.

Tissue Cult. Res. Commun. 18:329-338(1999)

PubMed=20143388; DOI=10.1002/ijc.25242

Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.

Check your cultures! A list of cross-contaminated or misidentified cell lines.

Int. J. Cancer 127:1-8(2010)