-GFPHuman umbilical vein endothelial cell GFP-

GFPP1HUVECsPuromycinGFP-HUVECs(P3)18

HUVECs were infected with GFP- expressing Lentiviral particles @ passage 1. Puromycin resistant GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below.

Medium Formulation：

ECGM Endothelial Cell Culture Medium（P1001）

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freeze storage conditions: 5% DMSO+95% FBS

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum) (SD0001)

Declaration: This product is for scientific research only.