C57BL/6Intestinal epithelial cells of C57BL / 6 mice-

C57BL/6 Mouse Primary Small Intestinal Epithelial Cells from AcceGen are isolated from small intestinal tissue of pathogen-free laboratory mice. C57BL/6 Mouse Primary Small Intestinal Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen's Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.

Medium Formulation:

Keratinocyte Serum-Free Medium KGM (P8001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum) (SD0001)

Declaration: This product is for scientific research only.