Tel:4001790116,13012892256,17521589219

Cell, culture reagent, life science research overall service provider!

Technical Support技术支持

CONTACT US

How Should We Deal With The "water Stress" In Cultured Cells

source:Qida organism  views:2343  time:2022-07-01

  & Nbsp; There Are Always A Lot Of Strange Things Happening Between Cells. For Example, For Cells In The Same Incubator, Why Others Are Good, But Mine Has Always Had Problems. They Either Pollute Or Die, Or Stagnate For A Short Time. They Change The Liquid Once Every Three Days, And Continue To Pass On The Cryopreservation Experiment. I Can't Change The Liquid Every Three Days, And I Can't Change The Liquid Every Day. How To Deal With The Adverse Water Phase& Nbsp;& Nbsp; & Nbsp; This Is A Cell On The Second Day Of Recovery. I Feel That There Is Really No Problem. I Always Feel That The Water Reversal Period Has Passed, And The Light Finally Comes. I Am Happy To Change The Liquid And Prepare For The Next Day's Generation& Nbsp; & Nbsp; However, After 24 Hours Of Observation Of The Cell State, It Turned Into The Following. All The Adherent Cells Were Clustered And Floated, And There Were Many Small Gravel Like Black Particles In The Background, Which Could Not Be Washed Away By PBS. It Was Obvious That The Cell Was Also Soaked& Nbsp; & Nbsp; If The Cells Are Contaminated By Bacteria, The State Of The Cells In The Culture Dish Or Bottle Will Change Quickly, Which Shows That A Large Number Of Particles Appear In The Cytoplasm, The Liquid Is Turbid, The PH Of The Culture Medium Drops, And Becomes Yellow, And The Adherent Cells Become Round In Shape And Will Die Of Desquamation; However, The State Of Cells Polluted By Fungi Will Not Change Immediately. After 3-4 Days Of Culture, You May See White Floating Objects Or Small Black Spots In The Culture Dish. There Are Only Two Possibilities, One Is Cell Pollution, And The Other Is That The Temperature Inside The Incubator Is Uneven, So You Need To Put Other Cells In That Position To Test& Nbsp; & Nbsp; However, It Is Strange That There Is Not Only One Person Raising This Cell In The Laboratory. Other People's Cells, Whether They Are The Same Or Other Cells, Are Very Good. Only This Student Recovers, Passes On, Or Borrows Cells Just From Others, Which Makes Her Afraid To Raise Any Cells For A Month. This Also Causes The Whole Laboratory To Help Her Find The Cause Of Pollution. Turn On Pollution Elimination & Nbsp; First Of All, We Use The Same Serum, Double Antibody And Culture Medium. If Any Of The Above Occurs, It Should Be All Human Cells That Have Problems. The Reagent Problem Should Be Ruled Out. Secondly, She Will Put The Consumables To Be Used In The Ultra Clean Table For Ultraviolet Irradiation For More Than 30 Minutes Before Each Use, So It Will Not Be The Pollution Caused By The Use Of Consumables, And The Problem Of Consumables Will Be Ruled Out& Nbsp; Finally, We Focused On The Intercellular Water Bath And Clothing. Because Recently, Except For This Unfortunate Classmate, No One Else Has Recovered Cells. The Student Moved The Water Bath Pan To The Tap In The Buffer Room For Convenience. The Water In The Water Bath Box Is The Tap Water In The Tap, And There Is A Dead Mosquito Floating In It Since The Student Entered The Cell, He Has Never Left His Big Earrings And Bracelets, Nor Washed His White Coat, Nor Shined His Cuffs. Even If The White Coat Between Cells Is Illuminated By Ultraviolet Light All The Year Round, He May Still Have Traces Of Pollution& Nbsp; & Nbsp; Therefore, Under Such Daily Operation, It Is Strange That Cells Will Not Die. In The Process Of Cell Culture, Ultrapure Water Is Required For Both Reagent Preparation And Water Bath For Cell Resuscitation; In Addition, Even If The Cryopreservation Tube Is Sealed With A Sealing Film, It Can Only Be Recovered In Ultra Pure Water& Nbsp; & Nbsp; At The Same Time, The Flow Of People Is Also One Of The Causes Of Uncontrollable Pollution Between Cells. The Flow Of Personnel Itself Will Carry A Lot Of Dust, And Bacteria Will Gather In The Dust. In Addition To Regular Cleaning And Replacement, Aseptic Clothing Between Cells Also Has Requirements On Clothing. Take A Bath And Change Clothes Frequently. It Is Better For Girls To Wear Less Jewelry. Methods For Handling Pollution In Cell Culture & Nbsp; If It Is Bacterial Pollution, Which Has Not Yet Broken Out, And The Pollution Is Relatively Small, We Can Add Our G/A And PS Respectively To The Culture Medium. Take 100ml Culture Medium As An Example: Add 0.75ml Of G/A, Add 0.5ml Of PS;, Change The Fluid After 24 Hours. After Two Consecutive Days Of Killing, The Black Spots Of Cells Decreased, And Then The Normal Concentration Of Antibiotics Was Returned& Nbsp; & Nbsp; Sometimes Several Vacuoles Can Be Seen In The Cytoplasm Of Some Cultured Cell Lines Under The Microscope. Some People Say That Because The Cells Are Different, The Morphology And Characteristics Are Also Different. For Example, Endothelial Cells Are A Kind Of "dirty" Cells. Because Of The Extrusion And Superposition Between Cells During The Culture Process, Many Round Structures Can Be Seen Visually. This Situation Is Different. There Are Many Vacuoles In The Cytoplasm And Nucleus, Which Is An Obvious Abnormality. However, Even Though The Color Of The Medium Has Not Changed, The Cells Continue To Divide And Grow, But The Morphology Changes And Lengthens& Nbsp; & Nbsp; In This Case, It Can Be Basically Determined That It Is Mycoplasma Contamination. It Is More Difficult To Find Cells Polluted By Mycoplasma Than By Fungi. At The Initial Stage, No Change Will Occur To The Cells, And There Will Be No Difference In The Color And Cell State Of The Culture Medium. After You Pass It On For Several Times, You Will Find That The Cell Grows Slowly And Its Shape Begins To Age. If We Do Not Carefully Observe The Changes Inside The Cells, We Can Easily Attribute Them To Cell Aging Caused By Improper Personal Treatment. This Also Reminds Us That The Daily Observation Of The State Of Cells Is Not Just About Whether There Are Cells Dying& Nbsp; & Nbsp; In The Cells Polluted By Mycoplasma, Vacuoles Of Different Sizes Can Be Seen In The Nucleus Or Cytoplasm Under High Power Microscope, And Cells Often Show A Sudden Sudden Death, But It Is Difficult To Find The Specific Stage Of Mycoplasma Infection. Therefore, Even If The Cells Are Frozen During This Period, There Will Still Be Mycoplasma Contamination After They Are Resuscitated. Mycoplasma Contamination Will Not Only Affect The Cell Morphology And Growth Speed, But Also Change The Characteristics Of The Cell Itself And Induce Changes In Chromosomes. Therefore, Even If The Cells Are Not Dead, It Will Also Have An Impact On The Experimental Results& Nbsp; & Nbsp; It Is Recommended To Use Mycoplasma Scavenger (Qida Biological, Product No.: SD0033), Which Is Prepared By Combining Small Molecule Antibiotics And Broad-spectrum Antibiotics, To Remove More Than 99% Of Different Mycoplasma Contamination. Mycoplasma Contamination Is Different From Bacterial Or Fungal Contamination. Once Mycoplasma Contamination Is Found, It Is Likely To Be An Overall Problem Of An Incubator Or A Laboratory, Rather Than A Personal Situation. Therefore, In Order To Prevent The Contamination And Spread Of Mycoplasma, The Best Way Is To Immediately Isolate The Discovered Cells And Clean And Disinfect The Incubator And Cells& Nbsp; & Nbsp; There Is Also The Black Glue Insect Pollution. As With The Discovery Of Mycoplasma Pollution, The Laboratory And Cell Culture Room Need To Be Quickly Disinfected. After That, The Precious Cells Are Isolated And Cultured With The Black Glue Insect Inhibitor (Qida Biological, Product No.: SD0036)& Nbsp; & Nbsp; In Addition To The Three Problems Mentioned Above, There Is Also Force Majeure Between Cells - Some People Will Die Of Any Cell They Raise At A Certain Time. After Checking All Suspicious Points, No Cause Of Pollution Can Be Found. If This Is The Case, It May Be The Time For Water Reversal. I Suggest That You Arrange Something Else For Yourself To Do, And Then You Can Grow Cells After That. Maybe There Will Be No Problem.
×