The New Star Of Endothelial Cell Experiment Is Coming

The New Comer Of Endothelial Cell Experiment Is Coming. HECV Human Umbilical Vein Endothelial Cells Are Coming. HECV Cells Are A Rare Immortalized Endothelial Cell Line. They Can Be Propagated 4-5 Times A Week Before Generation, And Can Be Multiplied By 5 Times In 8-12 Hours, Far Exceeding The Proliferation Speed And Morphology Of HMEC-1 Cell Line And HUVEC-T1. HECV Cells Are The Best Choice For Studying Endothelial Dysfunction, Blood Brain Barrier Experiment And Wound Healing Experiment. Cell Morphology Is As Follows:& Nbsp; 1、 Cell Identification Results: & Nbsp; 2、 The Wound Healing Test Was Used To Evaluate The Same Effect On HECV Cell Migration Ability: A) Multiple Groups Of Images Were Collected At 0, 6, 24 And 48 Hours To Determine The Cell Migration Under Different Conditions, And The Images Were Analyzed Using ImageJ Free Software (B) The Percentage Of Closed Area Was Measured And Compared With The Value Before Treatment. An Increase In The Percentage Of Restricted Areas Indicates Cells. The Data Are The Mean ± Standard Deviation Of Four Independent Experiments. Statistical Significance Between Groups Was Analyzed By ANOVA, And Then Tukey Test Was Performed& Nbsp; Conclusion: This Cell Has A Good Migration Detection Effect. In Endothelial Cells, Its Ability To Promote Endothelial Cell Migration May Be Important, Which Is An Important Basis For Reducing Or Improving Endothelial Dysfunction Under Pathological Conditions. Indeed, It Seems That The Initial Event In The Process Of Atherogenesis Is Some Form Of Damage Lining To The Intimal Endothelial Cells, Which Is Caused By Toxic Substances (such As Oxidized Cholesterol, Hyperglycemia, Etc.) Or Changes The Hemodynamics (such As Blood Flow Disorder Caused By Hypertension). In Particular, Focal Endothelial Cell Desquamation Is Considered To Stimulate Platelet Adhesion And Local Release Of Platelet-derived Growth Factor, And Then Trigger Migration, Proliferation And Phenotypic Regulation Of Medial Smooth Muscle Cells To Produce Plaque. In Conclusion, HECV Cells Are Reliable Models For Endothelial Cell Research In Vitro& Nbsp; Tips; Using ECGM Endothelial Cell Culture Medium Will Enable HECV Cells To Better Maintain The Growth Characteristics Of Endothelial Cells.