How To Select Cells When Doing Experiments With HepG2, Huh7 And Hep3B?

Hepatocellular Carcinoma (HCC) Is One Of The Malignant Tumors With A Rapidly Rising Incidence Rate In Recent Years. Hepatocellular Carcinoma Cell Lines Are Widely Used In The Study Of Various Types Of Liver Cancer. The Genome Of Hepatoma Cell Line Is Different From That Of Healthy Human Cell Line. Common Human Liver Cancer Cell Lines Include PLC, Huh7, Huh7.5, Hep3B, HepG2, SMMC-7721, MHCC97-H, MHCC9-L, Etc. Each Cell Line Has Its Own Characteristics. However, SMMC-7721, MHCC97-H, MHCC9-L, Etc. Have Been Removed From The Experimental Stage After Being Confirmed To Be Contaminated. Because They Have Similar Biological Characteristics With The Original Liver Cancer Cells, No Biological Changes, Stable Characteristics, And Unlimited Passage, They Provide An Ideal In Vitro Model For Liver Cancer Research, In Addition To Animal Experiments, It Has Become Another Popular Substitute For Liver Cancer Research& Nbsp; Huh-7 Cell Line Hidekazu Et Al. Isolated And Cultured Huh-7 From The Tissues Of Patients With Highly Differentiated Hepatocellular Carcinoma In 1982 [1], But The Genetic Map Of Hepatocellular Carcinoma Cells Is Not Known To The Public. Fumio Kasai Et Al. Carried Out M-Fish, SNP Microarray Sequence And Sequence Analysis On Huh7 Hepatoma Cell Line To Determine Its Map. They Found That High Level Of LOH Can Increase The Possibility Of Chromosome Rearrangement. Huh7 Hepatoma Cells Are Highly Sensitive To Genetic Variation In The Process Of Culture. In Addition, Different Generations May Have Different Expression Patterns In Vitro Experiments. Malinen Et Al. Found That The Long-term Differentiated Huh-7 Cell Line Is A Promising Model Of In Vitro Hepatotoxicity And Endogenous Compounds For Drug Detection And Other Experiments. Jouan Et Al. Found That HuH-7 Cells Upregulated Some Transporters Related To Farnesol X Receptor (FXR) And Nuclear Factor Erythrocyte 2 Related Factor 2 (Nrf2). These Data Suggest That It May Be Used To Study The Interaction Between Drugs And MRP When Expressing Some Major Hepatic Drug Transporters& Nbsp; Application: The Genome Characteristics Of Human Hepatoma Cell Line Huh7 Are Very Sensitive To The Number Of Generations. The Differentiated Huh7 Cells May Be Suitable For Studying The Hepatotoxicity Of Drug Metabolizing Enzyme Protein Expression. Huh-7 Cell Line Is Also Commonly Used To Study The Pathogenesis Of Hepatocellular Carcinoma. It Is A Convenient And Cheap Method To Replace Human Hepatocytes& Nbsp; Hep G2 Cell Line:; The Statistics Of HepG2 Cell Line Genome And Epigenome Related Literature Shows That The Genome Sequence Of HepG2 Hepatoma Cell Line Creates Higher Genomic Characteristics. It Fully Integrates The Whole Genome Characteristics And Gene Sequences Of HepG2 Cell Lines Including CN, SNPs, SNV, Etc., Laying A Foundation For The Whole Genome Identification Of Other Similar Cell Lines. Juliana Et Al Found That β- The Killing Effect Of Creatine On Human White Blood Cells Is More Obvious Than That On Human Liver Cancer Cells. The Hepatotoxicity Of Monoterpenoids Was Higher Than That Of HepG2/C3A Cells, And Better Than That Of Leukocytes. In Vivo Research Shows That β- The Carcinogenic And Teratogenic Effects Related To Muscle Strength Need Further Experimental Confirmation. Arunraj Et Al Found That The Composite Nano Gel Was Cytotoxic To HepG2 Cell Lines, And The Dox Chitin PLA CNG System May Be A Promising Anticancer Drug Delivery System For Liver Cancer  HepG2, A Human Hepatoma Cell Line, Has Been Widely Used In The Study Of Liver Physiology, But The Expression Levels Of Liver Specific Drug Metabolizing Enzymes And Transcription Factors Are Low& Nbsp; The Establishment Of Hep3B Hepatoma Cell CDNA Library Of Hep3B Cells And The Study Of Its Toxicity To Hep3B Hepatoma Cells Will Help Us Better Understand Liver Cancer And Select The Best Cell Line For Cell Experiments. Therefore, Human Hep3B Hepatoma Cell Line Has Been Used For A Long Time To Detect The Efficacy Of Therapeutic Drugs, In Vitro Cytotoxicity, DNA Synthesis And Caspase Activity  Human Liver Cancer Cells Mainly Come From Liver Cancer Tissue. By Culturing Liver Cancer Cells And Analyzing Them At The Single Cell Level, We Can Understand The Process Of Liver Cancer Formation In More Detail And Apply Them To The Biological Research Of Liver Cancer. One Advantage Of Using In Vitro Systems For Research Is That They Can Be Faster And Cheaper Than In Vivo Research. For Example, If You Want To Do An Experiment On The Proliferation Of Liver Cancer Cells, You Can Choose HepG2 Or Hep3B Liver Cancer Cells Instead Of Spending Too Much Time Choosing Them. The Genome And Cytotoxicity Of HepG2, Huh7 And Hep3B Have Been Studied Deeply, And These Genes Are Of Great Significance For The Development Of Liver Cancer And The Development Of Advanced Drugs& Nbsp; Extract From The Official Website Of ACCEGEN.