Cell Transfection Is Low. Do You Want To Use All The Transfection Reagents?

At Present, Cell Transfection Technology Is Mainly Divided Into Three Categories: Chemical Method, Physical Method And Biological Method. However, No Method Is Universal And Accurate, So Only By Selecting The Ideal Method According To Your Own Experimental Requirements Or Experimental Exploration Can You Obtain Beautiful Experimental Results. Of Course, You Can Also Read More About The Literature Published By The Predecessors, Which Can Reduce Many Unnecessary Costs. Is Low Cell Transfection A Problem With PCR Reagents? Insufficient Purity Of PCR Does Cause Such Problems, So It Is Important To Select PCR Reagents Mainly Because Of The Following Factors: 1; Other Microorganisms Pollute The Cells You Think You Raise. No, No, No, Maybe You Only Know That You Raise Cells. What You Don't Know Is That Your Cells May Have A Group Of Mistresses Behind Your Back, Such As Mycoplasma, Viruses, And Gumworms. In Particular, Mycoplasma Can Change The Characteristics Of Cells, Penetrate The Cell Membrane, And Carry Out Genetic Transformation. After Several Generations Of Culture, You May Not See The Cosmetic Effect Of "it" On Cells, But With The Increase Of Generations, The Cells Gradually Show That The "it" Has Become Not The "it" Of The Past. Therefore, It Is Recommended To Carry Out A Mycoplasma Detection Before Transfection. If Any Mycoplasma Is Removed, It Can Be Transfected Again. 2. Cross Contamination: If A Lot Of Cells Are Raised At The Same Time, And The Operation Is Not Standardized, Then A Cells Are Accidentally Sent To B Cells' Home To Have A Relative. The Final Result May Be That A Cells And B Cells Will Not Leave Each Other. If B Cells Are Strong, The Dove May Occupy The Nest. Therefore, We Must Pay Attention To The Operation. In Case Of Unclear Cross Contamination, We Would Rather Kill A Thousand By Mistake Than Let One Go. 3. Whether The Transfection Is High Or Not Is Very Important. It Is Not So Easy To Transfect Cells. It Can Be Done At Any Time, Just Like Not Having A Rush Of Urine Or Taking Up A Toilet. Compared With Non Dividing Cells, Dividing Cells Tend To Be Easier To Ingest And Express Foreign DNA Than Static Cells. Therefore, For Most Operations, Cells Are Generally Recommended To Plant Plates On The Day Of Transfection Or The Day Before. It Should Be Noted That There Should Not Be Too Many Planking Cells, In Particular, Cancer Cells Grow So Fast That If There Are Many Cells, Even If They Overlap With Each Other, The Cells Themselves Will Not Be Able To Eat Enough And Live Well. It Is Also Very Bad. How Can We Have Time To Play Transfection With You? 4.  The Transfection Effect Of Antibiotic And Serum Bias Was Originally Intended To Make It Difficult For Cells To Grow Well. In Order To Prevent The Possible Pollution Of Cells, They Added Various Antibiotics To Prevent Them, Such As Penicillin And Streptomycin. When Cells Are Transfected, If These Two Components Are Contained, The Permeability Of Cells Can Be Increased, Resulting In Low Transfection Or Cell Death. Therefore, Cells Cannot Speak, If You Can Speak, You Must Scold Us. Give Them All Kinds Of Medicine. Some Transfection Techniques, Such As Liposome Transfection, Are Inefficient In The Presence Of Serum, So Serum Should Be Removed Before Transfection. Cell Systems Primary Cells Are Washed And Centrifuged Without Antibodies, Which Can Make Cell Experiments More Accurate. The Influence Factors Published By Their Cells Are More Than 10 Points. In Addition, Impure DNA, Taking Relatives To Battle Or Excessive Endotoxin Will Lead To Low Transfection Effect Or Cell Death. It Is Important To Choose Brand Reagents. Some References Of HighQu PCR Reagents: 1. Disarrangement Of Endoplasmic Reticulummitochondria Communication Concerns Ca2+homeostasis In FRDA BioRxiv, 2020 LR Rodr í Guez, P CalapQuintana QPCR With ORA QPCR Green ROX L Mix (HighQu, Kralchtal, Germany) On A CFX ConnectTM Real Time PCR Detection System (Bio Rad); 2. . Disruption Of Neural Crest Enhancer Landscapes As An Etiological Mechanism For Human Neurocristopathies 2019, Kups.ub.unikoeln.de M Bartusel Real-time Quantitative PCR Was Performed On The Light Cycler 480 II (Roche) With The ORA QPCR Green ROX L Mix (HighQu).& Nbsp; 3.  A Combined Computational Pipeline To Detect Circular RNAs In Human Cancer Cells Under Hypoxic Stress Ournal Of Molecular …, 2019 A Di Liddo RT-qPCR Using 2X ORA QPCR Green ROX L Mix (highQu GmbH), …in A PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific)