Fungi In Cell Culture, Prevention And Treatment Of Cell Pollution

The Winter Has Passed For Most Of The Time, And Spring Is Coming. The Little Friends In The Laboratory On The First Floor, Basement Or Other Places Prone To Moisture Will Start A New Round Of Anti Cell Pollution War. Please Observe The Eternal Law Of Cell Pollution: As Long As Any Cell Is Polluted By Unimportant Cells, Add 84 And Discard Them Immediately, And Then Resuscitate And Culture In A New Frozen Tube; Please Refer To The Following For What Really Needs To Be Saved: Bacterial Pollution: Mainly The Onion Halbert Type Pollution Is Determined To Be Very Precious Cells. It Is Recommended To Prepare A PBS Containing 10 Times Gentamicin And Amphotericin Solution (G/A) And A Complete Culture Medium Containing 5 Times Gentamicin And Amphotericin Solution (G/A) Respectively. Then Wash The Cells 5-10 Times With PBS With 10 Times Of Double Antibody, And Then Wash The Cells With Complete Culture Of 5 Times Of Double Antibody For More Than 3 Times. Then Put The Cells In The Incubator With Double G/A Solution For Culture. Change The Solution After An Hour. After 4 Hours, Add The Antibiotics Required For Normal Cell Culture, And Stay Overnight. Finally, It Is Determined That The Cells Are Free Of Any Pollutants, So The Cells Are Damaged Too Much, It Is Suggested To Optimize The Culture Medium (increase Serum By 2% - 5%) Or Freeze Cells Immediately And Resuscitate After One Month. Fungal Pollution: Aspergillus, Candida Albicans, Yeast, Black Mold, Spore Fungus Are The Main Pollutants. Fungi Mainly Focus On Prevention, Supplemented By Prevention And Control, And Have Been Polluted. The Following Methods Are Recommended: 1; Once The Cells Are Polluted, It Is Difficult To Save Them. Even If Nystatin Or Actinomycin D Is Used, It Is Also A Way To Damage The Enemy By 8000 And Self Damage By 10000. Antibiotics Can Be Used To Kill Them. However, High Concentrations Of Antimycin Have Toxic Effects On Cells. 2.  Transfer All Cells From The Contaminated CO2 Incubator, And Scrub The Incubator With Peracetic Acid (all, Especially The Four Corners). Put Peracetic Acid In The Incubator For One Hour To Diffuse Its Vapor. After The Smell Of Peracetic Acid Is Dissipated, It Is Transferred Into Cells; 3.  Wipe The Super Clean Table And Wall With 0.5% Bromogeramine To Ensure That No Dead Space Is Left, And Mop The Floor With Bromogeramine Disinfectant. 4.  Thoroughly Disinfect The Environment, Fumigate The Entire Incubation Room With Potassium Permanganate Or Lactic Acid, Scrub The Incubator Interior Including Trays With Phenol, And Seal It For 2 Days. Fungal Pollution Prevention Methods: 1) Remove The Cells, Wipe The CO2 Incubator With A Trace Of Copper Sulfate Solution, And Then Add An Appropriate Amount Of Copper Sulfate Or Disodium Hydrogen Sulfate High Salt Liquid To The Water Pan To Prevent Mold 2) The Incubator Should Be Cleaned Regularly, About Once A Month, 3) Amphotericin Or Nystatin) Or [actinomycin D Or Double Antibody Can Be Added In The Culture Medium For Prevention. 4) The Most Important Thing Is To Cultivate A Good Aseptic Concept. The Laboratory Should Try Not To Speak Loudly, Wear Masks And Gloves, And Be Careful During Operation To Avoid The Medium From Being Spilled Into The Incubator And Biosafety Cabinet. These Media Spilled Into The Incubator Provide A Good Condition For The Propagation Of Bacteria And Fungi.