Immunohistochemical Procedure Price And Attention Details

Immunohistochemistry Is The Application Of The Basic Principle Of Immunology - Antigen Antibody Reaction, That Is, The Principle Of The Specific Combination Of Antigen And Antibody, To Determine The Antigens (polypeptides And Proteins) In Tissues And Cells Through The Color Development Of The Chromogenic Agents (fluorescein, Enzyme, Metal Ions, Isotopes) That Label Antibodies Through Chemical Reaction, And To Conduct Localization, Qualitative And Relative Quantitative Research On Them, It Is Called Immunohistochemistry Or Immunocytochemistry. 1、 Slide Washing  2、 II. Embedding Tissues With Paraffin  3、 The Embedded Tissue Sections, Generally 5 Um, Were Placed In 40 ℃ Warm Water After The Sectioning. 4. Salvage The Tissue. 5. Dewaxing. Put The Glass Slides Into Each Reagent Of Xylene Xylene - 100% Alcohol - 100% Alcohol - 95% Alcohol - 90% Alcohol - 80% Alcohol - 70% Alcohol For 10 Minutes In Turn. If The Weather Is Cold, The Time Can Be Appropriately Extended. 6. The Common Repair Methods For Antigen Repair Are Divided Into High-pressure Heating Repair, Microwave Repair And Pancreatic Duct Repair From Strong To Weak. High Pressure Heating Repair Is Simple And Its Effect Is Superior To Other Repair Methods. Precautions For Antigen Repair: (1) The Tissue Cannot Be Dried. (2) The Selection Of Antigen Repair Methods Varies From Antibody To Antibody. (3) This Method Is Mainly Used For 10% Formalin Fixation And Paraffin Embedded Tissue. (4) During The Process From Antigen Repair To DAB Coloration, PBS Buffer 7 And Serum Are Required To Block These Sites In Order To Prevent The Non-specific Binding Of The First Antibody To The Tissue, Bovine Serum Or Goat Serum Can Be Used To Block These Sites. The Blocking Serum Usually Comes From The Same Secondary Antibody Animal, And The Blocking Time Is 10-30 Minutes At Room Temperature& Nbsp; 8、 Add One Anti Nine, Two Anti Ten, And SABC; 11、 Add Color Developing Agent (configuration Of Color Developing Agent: Add 1 Drop Of Color Developing Agent A In 1ml Water, Shake Up, Then Add 1 Drop Of Color Developing Agent B, Shake Up, Add 1 Drop Of Color Developing Agent C, Shake Up)& Nbsp; A：DAB  & Nbsp; B：H2O2  & Nbsp; C: Phosphoric Acid Buffer; 12、 Re Dyeing Rinse The Color Developed Tablets With Water For A Period Of Time, And Then Soak Them In Hematoxylin For Dyeing. Generally, Animal Tissues Take Half A Minute, And Plant Tissues Take 3-5 Minutes& Nbsp; 13、 Dewatering XIV. Precautions For The Operation Of Sealing Film: 1& Nbsp; The Time Of Hematoxylin Re Staining Needs To Be Explored, Especially The Positive Staining Is Also On The Nucleus& Nbsp; 2.  & Nbsp; DAB Color Development Time Needs To Be Optimized, And The Positive Staining Is Obvious But The Background Is Not Too Deep Under Microscope& Nbsp; 3.  & Nbsp; The Incubation Time And Antibody Concentration Need To Be Explored. In Particular, The First Antibody Should Be Incubated At 4 ℃ Overnight& Nbsp; 4.  & Nbsp; Section Dewaxing And Hydration Should Be Sufficient; When Adding Reaction Solution, The Tissue Shall Be Fully Covered; The Dry Cleaning Solution Is Thrown Before Each Liquid Addition, But The Drying Is Prevented; Try To Draw A Larger Circle With The Histochemical Stroke, Otherwise The Ink Will Repel The Liquid And Cause The Periphery Of The Sheet To Dry.