What Should I Prepare For The Cell Experiment?

1. Commonly Used Pipettes Include Ostwald Pipette, Pipette And Graduated Pipette 2 How To Use The Pipette Correctly? Answer: (1) Selection Principle: Proximity Principle (2) Use Of Pipette: Suction - Wiping - Adjusting - Releasing 3 How To Use The Micro Sampler Correctly? Answer: Turn Press Suction Wipe Press 4. How To Use The Centrifuge Correctly? Placing - Piping - Balancing - Starting - Taking Out 5 What Are The Necessary Equipment For Cell Culture? Answer: The Necessary Equipment For Cell Culture Includes Ultra Clean Workbench, CO2 Incubator, Inverted Microscope, Liquid Nitrogen Biological Container, Pressure Steam Sterilizer, Sterile Filter, Electric Drying Oven, Centrifuge, Cell Counting Board, Refrigerator, Etc. 6. What Does Aseptic Operation Technology Include? Answer: 1. Keep A Quiet And Clean Sterile Working Area; 2. Keep The Working Surface Clean And Tidy; 3. Pay Attention To Personal Hygiene; 4. Aseptic Operation In A Clean Workbench 7. Experiment 1: Passage Culture Of Adherent Cells 7.1. What Is Adherent Cells? A: Adherent Cells, Also Known As Anchor Dependent Cells, Refer To Cells That Grow On The Surface Of Culture Vessels. Most Of The Cultured Cells Belong To This Category. Cell Attachment Or Adhesion To The Substrate Surface, Showing A Typical Morphological Process Of Polar Cells, Called Adherence. Adherence Is One Of The Conditions For The Growth And Proliferation Of Adherent Cells. 7.2. What Is The Experimental Principle Of Subculture Of Adherent Cells? A: Cell Adhesion Is Achieved Through The Combination Of Specific Cell Surface Receptors And Extracellular Matrix Molecules. Before Cell Spreading, Cells Have Secreted Extracellular Matrix And Proteoglycan. The Extracellular Matrix First Adheres To The Charged Culture Matrix, And Then The Cells Attach To The Matrix Through Specific Receptors. Protease Can Digest Extracellular Matrix, And Even Separate Monolayer Cultured Adherent Cells From Each Other By Degrading Extracellular Regions Of Transmembrane Proteins. Epithelial Cells Are Generally Not Easy To Dissociate, Because They Bind Cells Together Through Tight Junction Complexes; Mesenchymal Binding Is More Dependent On The Role Of Matrix, So It Is Easy To Separate. 7.3. What Is The Cell Counting Method? N/4 * 104 * Dilution Ratio 7.4. How To Identify Dead Cells And Living Cells? Trypan Blue Staining. 7.5. How To Count Cells? Answer: Under The Low Power Microscope, Count The Number Of Bright Cells In Each Grid Of The Four Corners Of The Board One By One In A Certain Direction. If The Cells Are Just Pressed On The Grid Line, Count Them According To The Principle Of Counting From Top To Bottom And From Left To Right.