Methods Of Endothelial Cell Culture And Preparation Of Culture Medium

Endothelial Cells Are An Indispensable Transmission Tool In Every Tissue, Including Malignant Tumors. At The Initial Stage, Blood Vessels First Expand A Wave To Attack Tissues In A Large Area. How Can Endothelial Cells Be Extracted And Cultured& Nbsp; The Primary Endothelial Cells Are Generally Separated By Tissue Enzyme Digestion. Don't Be Naive To Think That The Cells Digested By Enzymes Are Endothelial Cells, And There May Also Be Fibroblasts. This Involves How To Inhibit The Growth Of Fibroblasts And Let Endothelial Cells Grow Quickly. Endothelial Cells Generally Use Endothelial Cell Complete Culture Media (product Number: 4Z0-500) To Maintain Cell Growth. It Should Be Noted That Cells That Have Just Been Isolated Will Not Be Cultured In Serum-free Culture Media, Because Serum-free Culture Media May Cause Cells To Not Adhere To The Wall, And Serum-free Culture Methods Need To Be Replaced. Serum-free Components Can Only Be Replaced After Cells Are Stable, It Is More Suitable For Cell Growth Step By Step, Which Is Also The Reason Why Some Teachers Use Cell Coating Solution To Coat Culture Bottles When Extracting The Primary Generation& Nbsp; The Requirements For The Culture Medium Of Endothelial Cells Are Very High. Even The Cell Line HMEC-1 Must Use An Unconventional Basic Culture Medium, MCDB, And Then Add Serum, EGF, And Insulin To Grow Well. Therefore, If The Cell Culture Method Is Wrong, Efforts Will Be Wasted. Do Not Use A Very Radical Method To Change The Culture Method Of Any Cell, Because This Will Cause The Loss Of Some Genes Of The Cell And Lead To Cell Mutation; Endothelial Cell Culture Requires Frequent Fluid Change, Because They Secrete More, Especially When The Generation Is Not Good& Nbsp; One Of The Most Remarkable Characteristics Of Primary Endothelial Cells Is That When The Cell Generations Are Good, Their Digestion Time Is Very Short. It Seems That When Trypsin Is Added Here, They Need To Stop Immediately. They Are More Sensitive To Trypsin. However, Cells Or Endothelial Cell Lines With Too Many Passages Behave Differently. After Trypsin Is Added, They Can Come Down Just Like A Child Who Is Not Awake& Nbsp; Endothelial Microparticles Have The Function Of Transporters, RNA And Other Signal Molecules, And Play An Important Role In Regulating Thrombosis, Atherosclerosis, Inflammation, And Vascular Function. Endothelial Microparticles Express Many Endogenous Specific Molecules, Such As CD31, CD146, CD62E, CD51 CDl44. The Expression Of CD31 And CDl05 In Endothelial Microparticles Induced By Apoptosis Increases, And The Expression Of CD54 And CD62E In Endothelial Microparticles Induced By Activating Factors Increases; Under The Condition Of Promoting Thrombosis, The Expression Of Procoagulant Factor (TF) And Platelet Factor 3 On The Surface Of Endothelial Particles Increased. Because The Surface Of Endothelial Microparticles Carries A Lot Of Surface Proteins And Biological Information Of Endothelial Cells, The Expression Changes Under Different Pathological And Physiological Conditions, Which Is A Potential Marker For Non-invasive Monitoring Of Endothelial Cell Function& Nbsp; Endothelial Cells Are Also The Most Used Primary Cells In The Current Research, Which Is Inseparable From Its Function. Cell Systems Provides 37 Kinds Of Primary Endothelial Cells, And The Cells Do Not Use Antibodies To Sort Cells Without Resistance; The Number Of Cells In A Single Tube Reaches More Than 1 Million, Reducing The Funds For Your Experiment To Purchase Cells; The Most Important Thing Is To Supply The Coating Liquid In Domestic Stock, Which Can Greatly Shorten Your Waiting Time For Cells.