How To Digest Suspended Cells? Look Here

As We All Know, The Animal Cells Cultured In The Laboratory Can Generally Be Divided Into Two Categories: Adherent Cells And Suspension Cells. Adherent Cells Refer To The Cells That Can Grow And Reproduce On The Surface Of The Support That Can Be Attached And Only Depend On The Adhesion Factors Secreted By Themselves Or Provided In The Culture Medium. When Cells Grow On The Surface, They Will Generally Form Two Forms, Namely Fibroblast Like Cells Or Epithelioid Cells. The Other Is That The Cell Growth Does Not Depend On The Surface Of The Support, And It Grows In Suspension In The Culture Medium. This Type Of Cell Is Called Suspension Cell. When Observed Under The Microscope, The Suspension Cells Are Generally Round, Uniform In Size, Bright And Regular In Shape. Some Cells Also Gather And Grow In Clusters. In Fact, There Are Many Kinds Of Adherent Cells And Suspended Cells. When Cells In Vivo Are Cultured In Vitro, Most Of Them Grow By Adhering To The Wall, Mainly Including Some Normal Cells And Tumor Cells, Such As Fibroblasts, Bone Tissue (bone And Cartilage), Cardiac Muscle And Smooth Muscle, Liver, Lung, Kidney, Mammary Gland Skin Glial Cells, Endocrine Cells, Melanocytes, And Various Tumor Cells. While A Few Cells Grow In Suspension In Vitro, Including Some Cultured Cells From Blood, Spleen Or Bone Marrow, Especially White Blood Cells And Lymphocytes. Last Time, I Had A Brief Talk With My Friends About The Passage Method Of Adherent Cells. Today, Let's Discuss The Culture Of Suspension Cells. From The Definition Of Suspension Cells, Suspension Cells Are Non Adherent And Suspended Cells. Therefore, Suspension Cells Can Be Separated From The Surface Of The Culture Container Without The Action Of Enzymes. Therefore, The Method Of Subculture Of Suspension Cells Is Relatively Simple. In General, The Suspension Cells Are Usually Treated By Direct Passage And Centrifugal Passage (in Case Of Cell Fragments) In The Laboratory. When The Partners Observed That The Suspension Cells Had Grown To Full Size; When 80%~90% (cell Suspension Turns Yellow), Cells Can Be Subcultured. Under The Microscope, If The Cells Are In Good Condition (basically Without Cell Fragments And Regular Cell Morphology), The Direct Passage Method Can Be Selected. Divide The Culture Medium In The Original Bottle Into New Culture Bottles According To The Proportion (the Specific Proportion Depends On The Experiment, But It Cannot Be Too Thin, And Too Thin Cells Cannot Grow), And Then Add Fresh Culture Medium (the Amount Of Added Culture Medium Cannot Be Too Large, Otherwise Cells Will Not Be Able To Grow Because Of Low Density), And Then Observe The Cell Density Every Other Day To Consider Whether Fluid Replacement Is Required. However, When The Cell State Is Poor (there Are Many Cell Fragments And The Cell Shape Is Irregular) When Observed Under The Microscope, Centrifugation Passage Method Is Required. First, Transfer The Cell Suspension Into The Centrifuge Tube, 1000 Rpm; Centrifuge; 5 Min； Then Discard The Supernatant, Gently Scatter The Cell Sediment (try Not To Blow, Which Will Lead To Poor Cell Status Or Even Death), And Use Fresh Culture Medium To Resuspension Cells; Finally, Use A Straw To Absorb A Proper Amount Of Cell Suspension, Put It Into A New Culture Bottle, And Then Add A Proper Amount Of Fresh Medium To Continue The Culture. Well, Here We Are. See You Next Time.